Culturable endophytic fungi community structure isolated from Codonopsis pilosula roots and effect of season and geographic location on their structures

• Published in: BMC microbiology 2023-05, Vol.23 (1), p.132-132, Article 132
• Main Authors: Fan, Lili, Li, Yuanli, Wang, Xiaoli, Leng, Feifan, Li, Shaowei, Zhu, Ning, Chen, Kai, Wang, Yonggang
• Format: Article
• Language: English
• Subjects: Altitude; Analysis; Ascomycota; Autumn; Catalase; Caustic soda; Climatic conditions; Codonopsis - chemistry; Codonopsis pilosula; Community structure; Correlation analysis; Drug resistance in microorganisms; Endophytes; Endophytic fungi diversity; Environmental aspects; Environmental factors; Flowers & plants; Fungi; Geographical distribution; Geographical location; Geographical locations; Herbal medicine; Host plants; Longitude; Metabolites; Morphology; Mycobiome; Nutrients; Physiological aspects; Plant growth; Plant metabolites; Plant Roots - microbiology; Potassium; Rhizosphere; Roots; Seasonal variations; Seasons; Secondary metabolites; Soil; Soil characterization; Soil chemistry; Soil fungi; Soil microorganisms; Soil nutrients; Soil structure; Soils; Spatial distribution; Species composition; Spring; Spring (season); Summer; Temporal and spatial variation; Terrain; Urease; Winter
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-023-02848-3

Rhizosphere soil physicochemical, endophytic fungi have an important role in plant growth. A large number of endophytic fungi play an indispensable role in promoting plant growth and development, and they can provide protection for host plants by producing a variety of secondary metabolites to resist and inhibit plant pathogens. Due to the terrain of Gansu province is north-south and longitudinal, different climatic conditions, altitude, terrain and growth environment will affect the growth of Codonopsis pilosula, and the changes in these environmental factors directly affect the quality and yield of C. pilosula in different production areas. However, In C. pilosula, the connection between soil nutrients, spatiotemporal variation and the community structure of endophytic fungi isolated from C. pilosula roots has not been well studied. Seven hundred six strains of endophytic fungi were obtained using tissue isolation and the hyphaend-purification method from C. pilosula roots that picked at all seasons and six districts (Huichuan, HC; Longxi, LX; Zhangxian, ZX; Minxian, MX; Weiyuan, WY; and Lintao, LT) in Gansu Province, China. Fusarium sp. (205 strains, 29.04%), Aspergillus sp. (196 strains, 27.76%), Alternaria sp. (73 strains, 10.34%), Penicillium sp. (58 strains, 8.22%) and Plectosphaerella sp. (56 strains, 7.93%) were the dominant genus. The species composition differed from temporal and spatial distribution (Autumn and Winter were higher than Spring and Summer, MX and LT had the highest similarity, HC and LT had the lowest). physical and chemical of soil like Electroconductibility (EC), Total nitrogen (TN), Catalase (CAT), Urease (URE) and Sucrase (SUC) had significant effects on agronomic traits of C. pilosula (P