Development of new polymorphic microsatellite loci for the barley stem gall midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an enriched library

Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The o...

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Bibliographic Details
Published in:International journal of molecular sciences 2012-11, Vol.13 (11), p.14446-14450
Main Authors: Mezghani-Khemakhem, Maha, Bouktila, Dhia, Casse, Nathalie, Maaroufi, Houcine, Makni, Mohamed, Makni, Hanem
Format: Article
Language:English
Subjects:
Alleles
Barley
Cecidomyiidae
Cultivars
dinucleotide
Diptera
Genes
Genetic Loci
Genomics
Genotype
Hordeum - genetics
Hordeum vulgare
Mayetiola
Mayetiola destructor
Microsatellite Repeats - genetics
molecular ecology
Molecular Sequence Data
Nucleotide Motifs
Plant Stems
Polymorphism
Polymorphism, Genetic
trinucleotide
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Summary:Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population genetic studies on M. hordei.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms131114446