A Cell-Adhesive Plasma Polymerized Allylamine Coating Reduces the In Vivo Inflammatory Response Induced by Ti6Al4V Modified with Plasma Immersion Ion Implantation of Copper

Copper (Cu) could be suitable to create anti-infective implants based on Titanium (Ti), for example by incorporating Cu into the implant surface using plasma immersion ion implantation (Cu-PIII). The cytotoxicity of Cu might be circumvented by an additional cell-adhesive plasma polymerized allylamin...

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Bibliographic Details
Published in:Journal of functional biomaterials 2017-07, Vol.8 (3), p.30
Main Authors: Walschus, Uwe, Hoene, Andreas, Patrzyk, Maciej, Lucke, Silke, Finke, Birgit, Polak, Martin, Lukowski, Gerold, Bader, Rainer, Zietz, Carmen, Podbielski, Andreas, Nebe, J Barbara, Schlosser, Michael
Format: Article
Language:English
Subjects:
Addition polymerization
antibacterial properties
Antigen-presenting cells
Biocompatibility
Cell adhesion
Copper
Cytotoxicity
Distilled water
Immersion
Immunohistochemistry
In vivo methods and tests
Inflammation
Inflammatory response
Ion implantation
Lymphocytes
Lymphocytes T
Macrophages
Mast cells
morphometry
Neck
Photoelectron spectroscopy
Plasma
plasma immersion ion implantation
plasma polymerization
Plasmas (physics)
Polymerization
Rats
Spectroscopy
Surgical implants
Titanium
Titanium base alloys
Toxicity
Transplants & implants
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Summary:Copper (Cu) could be suitable to create anti-infective implants based on Titanium (Ti), for example by incorporating Cu into the implant surface using plasma immersion ion implantation (Cu-PIII). The cytotoxicity of Cu might be circumvented by an additional cell-adhesive plasma polymerized allylamine film (PPAAm). Thus, this study aimed to examine in vivo local inflammatory reactions for Ti6Al4V implants treated with Cu-PIII (Ti-Cu), alone or with an additional PPAAm film (Ti-Cu-PPAAm), compared to untreated implants (Ti). Successful Cu-PIII and PPAAm treatment was confirmed with X-ray Photoelectron Spectroscopy. Storage of Ti-Cu and Ti-Cu-PPAAm samples in double-distilled water for five days revealed a reduction of Cu release by PPAAm. Subsequently, Ti, Ti-Cu and Ti-Cu-PPAAm samples were simultaneously implanted into the neck musculature of 24 rats. After 7, 14 and 56 days, peri-implant tissue was retrieved from 8 rats/day for morphometric immunohistochemistry of different inflammatory cells. On day 56, Ti-Cu induced significantly stronger reactions compared to Ti (tissue macrophages, antigen-presenting cells, T lymphocytes) and to Ti-Cu-PPAAm (tissue macrophages, T lymphocytes, mast cells). The response for Ti-Cu-PPAAm was comparable with Ti. In conclusion, PPAAm reduced the inflammatory reactions caused by Cu-PIII. Combining both plasma processes could be useful to create antibacterial and tissue compatible Ti-based implants.
ISSN:2079-4983
2079-4983
DOI:10.3390/jfb8030030