Analysis of Bacteriohopanoids from Thermophilic Bacteria by Liquid Chromatography–Mass Spectrometry

Background: Hopanoids modify plasma membrane properties in bacteria and are often compared to sterols that modulate membrane fluidity in eukaryotes. In some microorganisms, they can also allow adaptations to extreme environments. Methods: Hopanoids were identified by liquid chromatography–mass spect...

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Bibliographic Details
Published in:Microorganisms (Basel) 2021-09, Vol.9 (10), p.2062
Main Authors: Kolouchová, Irena, Timkina, Elizaveta, Maťátková, Olga, Kyselová, Lucie, Řezanka, Tomáš
Format: Article
Language:English
Subjects:
Adaptation
Bacteria
Biosynthesis
Chromatography
Eukaryotes
Extreme environments
Fluidity
Geobacillus stearothermophilus
hopanoids
Lipids
Liquid chromatography
liquid chromatography–high-resolution electrospray mass spectrometry
Mass spectrometry
Mass spectroscopy
Membrane fluidity
Membranes
Microorganisms
Spectroscopy
Sterols
Strains (organisms)
Thermophilic bacteria
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Summary:Background: Hopanoids modify plasma membrane properties in bacteria and are often compared to sterols that modulate membrane fluidity in eukaryotes. In some microorganisms, they can also allow adaptations to extreme environments. Methods: Hopanoids were identified by liquid chromatography–mass spectrometry in fourteen strains of thermophilic bacteria belonging to five genera, i.e., Alicyclobacillus, Brevibacillus, Geobacillus, Meiothermus, and Thermus. The bacteria were cultivated at temperatures from 42 to 70 °C. Results: Regardless of the source of origin, the strains have the same tendency to adapt the hopanoid content depending on the cultivation temperature. In the case of aminopentol, its content increases; aminotetrol does not show a significant change; and in the case of aminotriol the content decreases by almost a third. The content of bacteriohopanetetrol and bacteriohopanetetrol glycoside decreases with increasing temperature, while in the case of adenosylhopane the opposite trend was found. Conclusions: Changes in hopanoid content can be explained by increased biosynthesis, where adenosylhopane is the first intermediate in the biosynthesis of the hopanoid side chain.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms9102062