Double-network hydrogel enhanced by SS31-loaded mesoporous polydopamine nanoparticles: Symphonic collaboration of near-infrared photothermal antibacterial effect and mitochondrial maintenance for full-thickness wound healing in diabetes mellitus

• Published in: Bioactive materials 2023-09, Vol.27, p.409-428
• Main Authors: Deng, Qing-Song, Gao, Yuan, Rui, Bi-Yu, Li, Xu-Ran, Liu, Po-Lin, Han, Zi-Yin, Wei, Zhan-Ying, Zhang, Chang-Ru, Wang, Fei, Dawes, Helen, Zhu, Tong-He, Tao, Shi-Cong, Guo, Shang-Chun
• Format: Article
• Language: English
• Subjects: Diabetic wound healing; Mesoporous polydopamine nanoparticles (MPDA NPs); Mitochondrial function maintenance; Photothermal antibacterial; SS31
• ISSN: 2452-199X; 2452-199X
• DOI: 10.1016/j.bioactmat.2023.04.004

Diabetic wound healing has become a serious healthcare challenge. The high-glucose environment leads to persistent bacterial infection and mitochondrial dysfunction, resulting in chronic inflammation, abnormal vascular function, and tissue necrosis. To solve these issues, we developed a double-network hydrogel, constructed with pluronic F127 diacrylate (F127DA) and hyaluronic acid methacrylate (HAMA), and enhanced by SS31-loaded mesoporous polydopamine nanoparticles (MPDA NPs). As components, SS31, a mitochondria-targeted peptide, maintains mitochondrial function, reduces mitochondrial reactive oxygen species (ROS) and thus regulates macrophage polarization, as well as promoting cell proliferation and migration, while MPDA NPs not only scavenge ROS and exert an anti-bacterial effect by photothermal treatment under near-infrared light irradiation, but also control release of SS31 in response to ROS. This F127DA/HAMA–MPDA@SS31 (FH–M@S) hydrogel has characteristics of adhesion, superior biocompatibility and mechanical properties which can adapt to irregular wounds at different body sites and provide sustained release of MPDA@SS31 (M@S) NPs. In addition, in a diabetic rat full thickness skin defect model, the FH–M@S hydrogel promoted macrophage M2 polarization, collagen deposition, neovascularization and wound healing. Therefore, the FH–M@S hydrogel exhibits promising therapeutic potential for skin regeneration. [Display omitted] •SS31 enhanced mitochondrial function, decreased ROS leakage and inhibited inflammation.•MPDA NPs possessing excellent photothermal property developed for application in anti-bacteria.•MPDA@SS31 NPs control release of SS31 in response to ROS with the oxidation of MPDA NPs.•F127DA/HAMA-MPDA@SS31 (FH-M@S) hydrogel showed excellent mechanical properties.•FH-M@S hydrogel showed potential to promote diabetic wound healing.