Antimicrobial activity and partial chemical structure of acylpolyamines isolated from the venom of the spider Acanthoscurria natalensis

Acylpolyamines are one of the main non-peptide compounds present in spider venom and represent a promising alternative in the search for new molecules with antimicrobial action. The venom of spider was fractionated by reverse-phase liquid chromatography (RP-HPLC) and the antimicrobial activity of th...

Full description

Saved in:
Bibliographic Details
Published in:The journal of venomous animals and toxins including tropical diseases 2022, Vol.28, p.e20210017-e20210017
Main Authors: Barth, Tania, Silva, Aline, Dos Santos, Simone Setubal, Santos, Jane Lima, Andrade, Patrícia Diniz, Tsai, Jessica, Caldas, Eloísa Dutra, Castro, Mariana de Souza, Pires, Osmindo Rodrigues
Format: Article
Language:English
Subjects:
Acanthoscurria natalensis
Acylpolyamines
Antimicrobial
Mass spectrometry
Spider venom
TOXICOLOGY
TROPICAL MEDICINE
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acylpolyamines are one of the main non-peptide compounds present in spider venom and represent a promising alternative in the search for new molecules with antimicrobial action. The venom of spider was fractionated by reverse-phase liquid chromatography (RP-HPLC) and the antimicrobial activity of the fractions was tested using a liquid growth inhibition assay. The main antimicrobial fraction containing acylpolyamines (ApAn) was submitted to two additional chromatographic steps and analyzed by MALDI-TOF. Fractions of interest were accumulated for ultraviolet (UV) spectroscopy and ESI-MS/MS analysis and for minimum inhibitory concentration (MIC) and hemolytic activity determination. Five acylpolyamines were isolated from the venom with molecular masses between 614 Da and 756 Da, being named ApAn728, ApAn614a, ApAn614b, ApAn742 and ApAn756. The analysis of UV absorption profile of each ApAn and the fragmentation pattern obtained by ESI-MS/MS suggested the presence of a tyrosyl unit as chromophore and a terminal polyamine chain consistent with structural units PA43 or PA53. ApAn presented MIC between 128 µM and 256 µM against and , without causing hemolysis against mouse erythrocytes. The antimicrobial and non-hemolytic properties of the analyzed ApAn may be relevant for their application as possible therapeutic agents and the identification of an unconventional chromophore for spider acylpolyamines suggests an even greater chemical diversity.
ISSN:1678-9199
1678-9199
DOI:10.1590/1678-9199-JVATITD-2021-0017