Physicochemical, Antimicrobial, Lethality and In Vitro Antioxidant Profiles of Johnu Tisane: A Coffee (Coffea Arabica) Leaf Decoction Formula

• Published in: Biology, medicine, & natural product chemistry (Online) medicine, & natural product chemistry (Online), 2024-05, Vol.13 (1), p.99-107
• Main Authors: Egbuonu, Anthony Cemaluk Chinedum, Alaebo, Prince Ogochukwu, Onuoha, Udumma Nsofor, Njoku, Chinomso Juliet, Obike, Chiemeziem Adanma, Nlemadim, Susan Ogechi, Chukwu, Blessing Ifeoma, Iwejuo, Sopuruchi Mary-Augusta, Amaechi, Onyedikachi Glory, Obiefuna, Victor Ifechukwu, Onuoha, Bessing Nmesomachi, Okoli, DivineTreasure Nzubechukwu, Nwokeoma, Precious Chinonso, Eze, Chukwubuikem
• Format: Article
• Language: English
• Subjects: in-vivo anti-oxidative outcomes and roles; johnu tisane; requisite anti-oxidant capacity; requisite physicochemical mix; rich phyto-constituents-related diverse pharmacologic activities
• ISSN: 2089-6514; 2540-9328
• DOI: 10.14421/biomedich.2024.131.99-107

Coffee leaves-based tea has relevance in ethno-medications due to its rich phyto-constituents-related diverse pharmacologic activities. Jonhu tisane, a typical Coffea arabica leaf decoction has no supporting scientific basis amidst reported location, processing and specie-related variations. This investigated physicochemical, antimicrobial, lethality and in-vitro antioxidant profiles of Johnu tisane by acceptable methods. Results recorded moisture (92.26 %), ash (0.65 %), unsaponified matter (1.46 mg/100 g), free fatty acid (0.56 mgKOH/g), acid value (1.12 mgKOH/g), potential hydrogen (6.85), lethal concentration (1000 ppm) and concentration-dependent antimicrobial activity. Anti-oxidation results revealed total antioxidant capacity, ferric reducing antioxidant power, nitric oxide, hydrogen peroxide and 2, 2-diphynyl-1-picrylhydrazyl scavenging activities increased concentration-dependently compared to standard. Thus, Johnu tisane demonstrated low minerals and keeping quality; requisite physicochemical mix for consumption and bioactivity; high safety margin; antimicrobial potency; and requisite anti-oxidation capacity for in-vivo antioxidant role. These provided scientific support for its ethno-medicinal uses. They underscored the need to elucidate its pharmacologically active compounds; mechanistic roles in animal models; and the impact of ash to moisture mix variation on potential hydrogen, microbial and antimicrobial activities in relation to shelf life, bioactivity and in vivo anti-oxidative roles for novel insights on preserving sample quality, safety, bioactivity and in-vivo anti-oxidative outcomes.