Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

A long wavelength TCF-based fluorescent probe ( ) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to pur...

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Bibliographic Details
Published in:Frontiers in chemistry 2019-04, Vol.7, p.255-255
Main Authors: Gwynne, Lauren, Sedgwick, Adam C, Gardiner, Jordan E, Williams, George T, Kim, Gyoungmi, Lowe, John P, Maillard, Jean-Yves, Jenkins, A Toby A, Bull, Steven D, Sessler, Jonathan L, Yoon, Juyoung, James, Tony D
Format: Article
Language:English
Subjects:
alkaline phosphatase
cell imaging
Chemistry
colorimetric
fluorescence
reaction-based fluorescent probe
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Summary:A long wavelength TCF-based fluorescent probe ( ) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.
ISSN:2296-2646
2296-2646
DOI:10.3389/fchem.2019.00255