Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants

• Published in: International journal of nanomedicine 2015-10, Vol.10 Suppl 1 (1), p.119-127
• Main Authors: Nithya, Rajendran, Meenakshi Sundaram, Nachiappan
• Format: Article
• Language: English
• Subjects: Animals; Bone and Bones - chemistry; Bone and Bones - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Ciprofloxacin - chemistry; Ciprofloxacin - pharmacology; Drug Delivery Systems - methods; Durapatite - chemistry; Humans; Mice; Nanocomposites - chemistry; NIH 3T3 Cells; Original Research; Osteoblasts - drug effects; Osteoblasts - metabolism; Polyesters - chemistry; Polymers - chemistry; Polymers - pharmacology; Prostheses and Implants; Topoisomerase II Inhibitors - chemistry; Topoisomerase II Inhibitors - pharmacology
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S79995

In recent years there has been a steep increase in the number of orthopedic patients for many reasons. One major reason is osteomyelitis, caused by pyrogenic bacteria, with progressive infection of the bone or bone marrow and surrounding tissues. So antibiotics must be introduced during bone implantation to avoid prolonged infection. The objective of the study reported here was to prepare a composite film of nanocrystalline hydroxyapatite (HAp) and polycaprolactone (PCL) polymer loaded with ciprofloxacin, a frequently used antibiotic agent for bone infections. Nanocrystalline HAp was synthesized by precipitation method using the precursor obtained from eggshell. The nanocomposite film (HAp-PCL-ciprofloxacin) was prepared by solvent evaporation. Drug-release and biodegradation studies were undertaken by immersing the composite film in phosphate-buffered saline solution, while a cytotoxicity test was performed using the fibroblast cell line NIH-3T3 and osteoblast cell line MG-63. The pure PCL film had quite a low dissolution rate after an initial sharp weight loss, whereas the ciprofloxacin-loaded HAp-PCL nanocomposite film had a large weight loss due to its fast drug release. The composite film had higher water absorption than the pure PCL, and increasing the concentration of the HAp increased the water absorption. The in vitro cell-line study showed a good biocompatibility and bioactivity of the developed nanocomposite film. The prepared film will act as a sustainable bone implant in addition to controlled drug delivery.