Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA

Thousand-grain weight (TGW) of wheat ( L.) contributes significantly to grain yield. In the present study, a candidate gene associated with TGW was identified through specific-locus amplified fragment sequencing (SLAF-seq) of DNA bulks of recombinant inbred lines (RIL) derived from the cross between...

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Published in:Frontiers in plant science 2016-12, Vol.7, p.1902-1902
Main Authors: Hu, Ming-Jian, Zhang, Hai-Ping, Liu, Kai, Cao, Jia-Jia, Wang, Sheng-Xing, Jiang, Hao, Wu, Zeng-Yun, Lu, Jie, Zhu, Xiao F, Xia, Xian-Chun, Sun, Gen-Lou, Ma, Chuan-Xi, Chang, Cheng
Format: Article
Language:English
Subjects:
Common wheat
Gene Expression
Gene-specific marker
IAA
Plant Science
SLAF-seq
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Summary:Thousand-grain weight (TGW) of wheat ( L.) contributes significantly to grain yield. In the present study, a candidate gene associated with TGW was identified through specific-locus amplified fragment sequencing (SLAF-seq) of DNA bulks of recombinant inbred lines (RIL) derived from the cross between Jing 411 and Hongmangchun 21. The gene was located on chromosome 7A, designated as with a complete genome sequence and an open reading frame (ORF). A single nucleotide polymorphism (SNP) was present in the first exon between two alleles at locus, resulting in a Val to Ala substitution, corresponding to a change from higher to lower TGW. Cleaved amplified polymorphic sequence (CAPS) ( ) and InDel ( ) markers were developed to discriminate the two alleles and for higher and lower TGW, respectively. A major QTL co-segregating with explained 21.7-27.1% of phenotypic variance for TGW in the RIL population across five environments. The association of with TGW was further validated in a natural population and Chinese mini-core collections. Quantitative real-time PCR revealed higher transcript levels of than those of during grain development. High frequencies of the superior allele for higher TGW in Chinese mini-core collections (65.0%) and 501 wheat varieties (86.0%) indicated a strong and positive selection of this allele in wheat breeding. The molecular markers and can be used for improvement of TGW in breeding programs.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2016.01902