Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography

Periplocosides, which are insecticidal compounds isolated from the root bark of Periploca sepium Bunge, can affect the digestive system of insects. However, the mechanism though which periplocosides induces a series of symptoms remains unknown. In this study, affinity chromatography was conducted by...

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Bibliographic Details
Published in:Toxins 2016-05, Vol.8 (5), p.139-139
Main Authors: Feng, Mingxing, He, Zhenyu, Wang, Yuanyuan, Yan, Xiufang, Zhang, Jiwen, Hu, Zhaonong, Wu, Wenjun
Format: Article
Language:English
Subjects:
affinity chromatography
Animals
binding protein
Carrier Proteins - isolation & purification
Chromatography, Affinity
Digestive system
Esters
Insect Proteins - isolation & purification
Insecticides
Intestines - chemistry
Larva
Larvae
Lepidoptera
Liquid chromatography
Luciferin
Mass spectrometry
Microvilli
Mythimna separata
Noctuidae
Oligosaccharides
Periploca
periplocosides E
Pregnenes
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Summary:Periplocosides, which are insecticidal compounds isolated from the root bark of Periploca sepium Bunge, can affect the digestive system of insects. However, the mechanism though which periplocosides induces a series of symptoms remains unknown. In this study, affinity chromatography was conducted by coupling periplocoside E-semi-succinic acid ester with epoxy amino hexyl (EAH) sepharose 4B. Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) was performed to analyze the fraction eluted by periplocoside E. Eight binding proteins (luciferin 4-monooxygenase, aminopeptidase N, aminopeptidase N3, nicotinamide adenine dinucleotide health (NADH) dehydrogenase subunit 5, phosphatidylinositol 3-phosphate 3-phosphatase myotubularin, actin, uncharacterized family 31 glucosidase KIAA1161, and 2OG-Fe(2) oxygenase superfamily protein) were obtained and identified through liquid chromatography/quadrupole-time of flight-mass spectrometry (LC/Q-TOF-MS) analysis of the midgut epithelium cells of Mythimna separata larvae. Aminopeptidase N and N3 are potential putative targets of periplocosides. This study establishes the foundation for further research on the mechanism of action and target localization of periplocosides in agricultural pests.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins8050139