Effect of titanium surface on secretion of IL1β and TGFβ1 by mononuclear cells

Mononuclear cells play an important role in the modulation of healing. The characteristics of implant surface topography may alter the production of signaling molecules such as cytokines. The aim of this in vitro study was to evaluate the effects of commercially available titanium surface treatments...

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Bibliographic Details
Published in:Brazilian oral research 2011-12, Vol.25 (6), p.500-505
Main Authors: Moura, Camilla Christian Gomes, Soares, Priscilla Barbosa Ferreira, Souza, Maria Aparecida de, Zanetta-Barbosa, Darceny
Format: Article
Language:English
Subjects:
Analysis of Variance
Blood Cells - secretion
Cell Survival
Cells, Cultured
Dentistry
DENTISTRY, ORAL SURGERY & MEDICINE
Enzyme-Linked Immunosorbent Assay
Humans
In vitro
Interleukin-1
Interleukin-1beta - secretion
Statistics, Nonparametric
Surface Properties
Time Factors
Titanium
Titanium - chemistry
Transforming Growth Factor Beta
Transforming Growth Factor beta1 - secretion
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Summary:Mononuclear cells play an important role in the modulation of healing. The characteristics of implant surface topography may alter the production of signaling molecules such as cytokines. The aim of this in vitro study was to evaluate the effects of commercially available titanium surface treatments on both cell viability and the secretion of the antagonist cytokines, IL1β and TGFβ1. Human mononuclear cells were cultured on 10 mm diameter commercially pure titanium (cpTi) disks that were prepared using a turning procedure (control = machined surface) and either acid etched or bio-anodized for 1-7 days. Adhered cells were investigated with respect to cell viability using an MTT assay, and cytokine production was verified using an ELISA assay. The results indicate that surface characteristics did not alter the cell viability at days 1 and 4, although the machined surface presented the highest absorbance values at day 7 (p = 0.0084). Cell viability was reduced throughout the time course for all analyzed surfaces (p < 0.05). On day 4, IL1β levels were significantly higher on bio-anodized compared to acid etched surfaces (p = 0.0097). TGFβ1 did not show differences among the surfaces at days 1 and 4. The responses of non-stimulated mononuclear cells to titanium surfaces suggest only modest effects of the surface treatment and roughness on pro-inflammatory cytokine (IL1β) release.
ISSN:1806-8324
1807-3107
1807-3107
1806-8324
DOI:10.1590/S1806-83242011000600005