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Molecular Characterisation of Cytochrome Oxidase I and Internal Transcribed Spacer 2 Fragments of Culiseta longiareolata
Among the mosquitoes, plays a notable role in the transmission of avian malaria, tularemia and arboviral diseases, including West Nile fever. We conducted this study to characterise the cytochrome oxidase I (COI) and internal transcribed spacer 2 (ITS2) fragments of in northwestern Iran to determine...
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| Published in: | Türkiye parazitolojii dergisi 2020-12, Vol.44 (4), p.191-196 |
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| container_title | Türkiye parazitolojii dergisi |
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| creator | Soltanbeiglu, Shadiyeh Vahedi, Mozaffar Mohammadi Bavani, Mulood Chavshin, Ali Reza |
| description | Among the mosquitoes,
plays a notable role in the transmission of avian malaria, tularemia and arboviral diseases, including West Nile fever. We conducted this study to characterise the cytochrome oxidase I (COI) and internal transcribed spacer 2 (ITS2) fragments of
in northwestern Iran to determine the classification status of this species.
The COI and ITS2 fragments from six populations of
were amplified, sequenced and analysed. For phylogenetic analysis, the evolutionary history was estimated using the Tamura-Nei-based Maximum Likelihood approach.
Thirteen sequences (six for ITS2 and seven for COI) from six populations of
were acquired and deposited into the GenBank. Phylogenetic analysis showed that the COI sequences from the current study cluster together with the same species from other parts of the world. Moreover, the ITS2 sequences of the current study and sequences retrieved from the GenBank, despite intraspecies variation, fall into a distinct clade with acceptable bootstrap values.
Notable genetic variations were observed between various
populations based on the evaluations of ITS2 and COI fragments. By conducting such studies, the exact classification status of this species can be determined. |
| doi_str_mv | 10.4274/tpd.galenos.2020.6886 |
| format | article |
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plays a notable role in the transmission of avian malaria, tularemia and arboviral diseases, including West Nile fever. We conducted this study to characterise the cytochrome oxidase I (COI) and internal transcribed spacer 2 (ITS2) fragments of
in northwestern Iran to determine the classification status of this species.
The COI and ITS2 fragments from six populations of
were amplified, sequenced and analysed. For phylogenetic analysis, the evolutionary history was estimated using the Tamura-Nei-based Maximum Likelihood approach.
Thirteen sequences (six for ITS2 and seven for COI) from six populations of
were acquired and deposited into the GenBank. Phylogenetic analysis showed that the COI sequences from the current study cluster together with the same species from other parts of the world. Moreover, the ITS2 sequences of the current study and sequences retrieved from the GenBank, despite intraspecies variation, fall into a distinct clade with acceptable bootstrap values.
Notable genetic variations were observed between various
populations based on the evaluations of ITS2 and COI fragments. By conducting such studies, the exact classification status of this species can be determined.</description><identifier>ISSN: 1300-6320</identifier><identifier>ISSN: 2146-3077</identifier><identifier>EISSN: 2146-3077</identifier><identifier>DOI: 10.4274/tpd.galenos.2020.6886</identifier><identifier>PMID: 33269558</identifier><language>eng</language><publisher>Turkey: Galenos Publishing House</publisher><subject>Animals ; Base Sequence ; Classification ; Culicidae - classification ; Culicidae - genetics ; Culiseta longiareolata ; Cytochrome ; Cytochrome oxidase I ; Disease transmission ; DNA, Ribosomal Spacer - genetics ; Electron Transport Complex IV - genetics ; Fever ; Genetic diversity ; Genetic Variation ; Insect Proteins - genetics ; Iran ; Likelihood Functions ; Malaria ; molecular systematics ; Mosquito Vectors - classification ; Mosquito Vectors - genetics ; mosquitoes ; phylogenetic analysis ; Phylogenetics ; Phylogeny ; Species ; Tularemia</subject><ispartof>Türkiye parazitolojii dergisi, 2020-12, Vol.44 (4), p.191-196</ispartof><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3656-210324f2ed9c08fe98f58f6ba35b5408c7c2ae08153686bf520c5e31093b443</citedby><orcidid>0000-0001-7543-8857 ; 0000-0002-2359-8610 ; 0000-0003-1590-1357 ; 0000-0002-1137-4572</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2474490520?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,37013,44590</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33269558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soltanbeiglu, Shadiyeh</creatorcontrib><creatorcontrib>Vahedi, Mozaffar</creatorcontrib><creatorcontrib>Mohammadi Bavani, Mulood</creatorcontrib><creatorcontrib>Chavshin, Ali Reza</creatorcontrib><title>Molecular Characterisation of Cytochrome Oxidase I and Internal Transcribed Spacer 2 Fragments of Culiseta longiareolata</title><title>Türkiye parazitolojii dergisi</title><addtitle>Turkiye Parazitol Derg</addtitle><description>Among the mosquitoes,
plays a notable role in the transmission of avian malaria, tularemia and arboviral diseases, including West Nile fever. We conducted this study to characterise the cytochrome oxidase I (COI) and internal transcribed spacer 2 (ITS2) fragments of
in northwestern Iran to determine the classification status of this species.
The COI and ITS2 fragments from six populations of
were amplified, sequenced and analysed. For phylogenetic analysis, the evolutionary history was estimated using the Tamura-Nei-based Maximum Likelihood approach.
Thirteen sequences (six for ITS2 and seven for COI) from six populations of
were acquired and deposited into the GenBank. Phylogenetic analysis showed that the COI sequences from the current study cluster together with the same species from other parts of the world. Moreover, the ITS2 sequences of the current study and sequences retrieved from the GenBank, despite intraspecies variation, fall into a distinct clade with acceptable bootstrap values.
Notable genetic variations were observed between various
populations based on the evaluations of ITS2 and COI fragments. By conducting such studies, the exact classification status of this species can be determined.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Classification</subject><subject>Culicidae - classification</subject><subject>Culicidae - genetics</subject><subject>Culiseta longiareolata</subject><subject>Cytochrome</subject><subject>Cytochrome oxidase I</subject><subject>Disease transmission</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Electron Transport Complex IV - genetics</subject><subject>Fever</subject><subject>Genetic diversity</subject><subject>Genetic Variation</subject><subject>Insect Proteins - genetics</subject><subject>Iran</subject><subject>Likelihood Functions</subject><subject>Malaria</subject><subject>molecular systematics</subject><subject>Mosquito Vectors - classification</subject><subject>Mosquito Vectors - genetics</subject><subject>mosquitoes</subject><subject>phylogenetic analysis</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Species</subject><subject>Tularemia</subject><issn>1300-6320</issn><issn>2146-3077</issn><issn>2146-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkU1r3DAQQEVpabZpf0KLoJdedivry_KxLE26kJJDchdjebTRIltbyYbk38fObnLoSSDePA16hHyt2EbyWv4cj91mDxGHVDaccbbRxuh3ZMUrqdeC1fV7sqoEY2stOLsgn0o5MKYV1-IjuRCC60YpsyKPf1NEN0XIdPsAGdyIORQYQxpo8nT7NCb3kFOP9PYxdFCQ7igMHd0NMzhApPcZhuJyaLGjd0dwmCmnVxn2PQ5jeXFMMRQcgcY07ANkTBFG-Ew-eIgFv5zPS3J39ft--2d9c3u92_66WTuhlV7zigkuPceuccx4bIxXxusWhGqVZMbVjgMyUymhjW694swpFBVrRCuluCS7k7VLcLDHHHrITzZBsC8XKe8t5DG4iBbmR4Tnuq3aWqI3BiXyVhrXdCAkW1w_Tq5jTv8mLKPtQ3EYIwyYpmK51LqulWzMjH7_Dz2kafmuhaqlbNi850ypE-VyKiWjf1uwYnaJbOfI9hzZLpHtEnme-3a2T22P3dvUa1XxDHebpe0</recordid><startdate>20201202</startdate><enddate>20201202</enddate><creator>Soltanbeiglu, Shadiyeh</creator><creator>Vahedi, Mozaffar</creator><creator>Mohammadi Bavani, Mulood</creator><creator>Chavshin, Ali Reza</creator><general>Galenos Publishing House</general><general>Galenos Yayinevi</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>EDSIH</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-7543-8857</orcidid><orcidid>https://orcid.org/0000-0002-2359-8610</orcidid><orcidid>https://orcid.org/0000-0003-1590-1357</orcidid><orcidid>https://orcid.org/0000-0002-1137-4572</orcidid></search><sort><creationdate>20201202</creationdate><title>Molecular Characterisation of Cytochrome Oxidase I and Internal Transcribed Spacer 2 Fragments of Culiseta longiareolata</title><author>Soltanbeiglu, Shadiyeh ; Vahedi, Mozaffar ; Mohammadi Bavani, Mulood ; Chavshin, Ali Reza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3656-210324f2ed9c08fe98f58f6ba35b5408c7c2ae08153686bf520c5e31093b443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Classification</topic><topic>Culicidae - classification</topic><topic>Culicidae - genetics</topic><topic>Culiseta longiareolata</topic><topic>Cytochrome</topic><topic>Cytochrome oxidase I</topic><topic>Disease transmission</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Electron Transport Complex IV - genetics</topic><topic>Fever</topic><topic>Genetic diversity</topic><topic>Genetic Variation</topic><topic>Insect Proteins - genetics</topic><topic>Iran</topic><topic>Likelihood Functions</topic><topic>Malaria</topic><topic>molecular systematics</topic><topic>Mosquito Vectors - classification</topic><topic>Mosquito Vectors - genetics</topic><topic>mosquitoes</topic><topic>phylogenetic analysis</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Species</topic><topic>Tularemia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soltanbeiglu, Shadiyeh</creatorcontrib><creatorcontrib>Vahedi, Mozaffar</creatorcontrib><creatorcontrib>Mohammadi Bavani, Mulood</creatorcontrib><creatorcontrib>Chavshin, Ali Reza</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Turkey Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Türkiye parazitolojii dergisi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soltanbeiglu, Shadiyeh</au><au>Vahedi, Mozaffar</au><au>Mohammadi Bavani, Mulood</au><au>Chavshin, Ali Reza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Characterisation of Cytochrome Oxidase I and Internal Transcribed Spacer 2 Fragments of Culiseta longiareolata</atitle><jtitle>Türkiye parazitolojii dergisi</jtitle><addtitle>Turkiye Parazitol Derg</addtitle><date>2020-12-02</date><risdate>2020</risdate><volume>44</volume><issue>4</issue><spage>191</spage><epage>196</epage><pages>191-196</pages><issn>1300-6320</issn><issn>2146-3077</issn><eissn>2146-3077</eissn><abstract>Among the mosquitoes,
plays a notable role in the transmission of avian malaria, tularemia and arboviral diseases, including West Nile fever. We conducted this study to characterise the cytochrome oxidase I (COI) and internal transcribed spacer 2 (ITS2) fragments of
in northwestern Iran to determine the classification status of this species.
The COI and ITS2 fragments from six populations of
were amplified, sequenced and analysed. For phylogenetic analysis, the evolutionary history was estimated using the Tamura-Nei-based Maximum Likelihood approach.
Thirteen sequences (six for ITS2 and seven for COI) from six populations of
were acquired and deposited into the GenBank. Phylogenetic analysis showed that the COI sequences from the current study cluster together with the same species from other parts of the world. Moreover, the ITS2 sequences of the current study and sequences retrieved from the GenBank, despite intraspecies variation, fall into a distinct clade with acceptable bootstrap values.
Notable genetic variations were observed between various
populations based on the evaluations of ITS2 and COI fragments. By conducting such studies, the exact classification status of this species can be determined.</abstract><cop>Turkey</cop><pub>Galenos Publishing House</pub><pmid>33269558</pmid><doi>10.4274/tpd.galenos.2020.6886</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-7543-8857</orcidid><orcidid>https://orcid.org/0000-0002-2359-8610</orcidid><orcidid>https://orcid.org/0000-0003-1590-1357</orcidid><orcidid>https://orcid.org/0000-0002-1137-4572</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Base Sequence Classification Culicidae - classification Culicidae - genetics Culiseta longiareolata Cytochrome Cytochrome oxidase I Disease transmission DNA, Ribosomal Spacer - genetics Electron Transport Complex IV - genetics Fever Genetic diversity Genetic Variation Insect Proteins - genetics Iran Likelihood Functions Malaria molecular systematics Mosquito Vectors - classification Mosquito Vectors - genetics mosquitoes phylogenetic analysis Phylogenetics Phylogeny Species Tularemia |
| title | Molecular Characterisation of Cytochrome Oxidase I and Internal Transcribed Spacer 2 Fragments of Culiseta longiareolata |
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