Anion channel SLAH3 is a regulatory target of chitin receptor-associated kinase PBL27 in microbial stomatal closure

In plants, antimicrobial immune responses involve the cellular release of anions and are responsible for the closure of stomatal pores. Detection of microbe-associated molecular patterns (MAMPs) by pattern recognition receptors (PRRs) induces currents mediated via slow-type (S-type) anion channels b...

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Bibliographic Details
Published in:eLife 2019-09, Vol.8
Main Authors: Liu, Yi, Maierhofer, Tobias, Rybak, Katarzyna, Sklenar, Jan, Breakspear, Andy, Johnston, Matthew G, Fliegmann, Judith, Huang, Shouguang, Roelfsema, M Rob G, Felix, Georg, Faulkner, Christine, Menke, Frank Lh, Geiger, Dietmar, Hedrich, Rainer, Robatzek, Silke
Format: Article
Language:English
Subjects:
Abscisic acid
Anion channels
Anions
Arabidopsis - drug effects
Arabidopsis - immunology
Arabidopsis Proteins - metabolism
Cellular signal transduction
CERK1
Channel pores
Chitin
Chitin - immunology
Fungi - chemistry
Gene Expression Regulation, Plant
Immune response
Ion channels
Ion Channels - metabolism
Kinases
LYK5
MAMP
Microbial colonies
Observations
PAMP-triggered immunity
Pattern recognition receptors
Peptides
Phosphatase
Phosphorylation
Physiological aspects
Plant Biology
Plant Stomata - drug effects
Plant Stomata - physiology
Protein Kinases - metabolism
Protein-Serine-Threonine Kinases - metabolism
Proteins
PTI
Receptors, Pattern Recognition - metabolism
SLAH3
Software
Stomata
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Summary:In plants, antimicrobial immune responses involve the cellular release of anions and are responsible for the closure of stomatal pores. Detection of microbe-associated molecular patterns (MAMPs) by pattern recognition receptors (PRRs) induces currents mediated via slow-type (S-type) anion channels by a yet not understood mechanism. Here, we show that stomatal closure to fungal chitin is conferred by the major PRRs for chitin recognition, LYK5 and CERK1, the receptor-like cytoplasmic kinase PBL27, and the SLAH3 anion channel. PBL27 has the capacity to phosphorylate SLAH3, of which S127 and S189 are required to activate SLAH3. Full activation of the channel entails CERK1, depending on PBL27. Importantly, both S127 and S189 residues of SLAH3 are required for chitin-induced stomatal closure and anti-fungal immunity at the whole leaf level. Our results demonstrate a short signal transduction module from MAMP recognition to anion channel activation, and independent of ABA-induced SLAH3 activation.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.44474