Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin B

• Published in: RGO - Revista Gaúcha de Odontologia 2019, Vol.67, p.e20190018
• Main Authors: MONTEIRO, Larissa Cavalcanti, RIBEIRO, Isabella Lima Arrais, BATISTA, Roberta Ferreti Bonan Dantas, MACIEL, Panmella Pereira, VALENÇA, Ana Maria Gondim, DULGHEROFF, Ana Carolina Bernardes, SOUZA, Joelma Rodrigues, CAVALCANTI, Yuri Wanderley, CASTELLANO, Lucio Roberto Cançado, BONAN, Paulo Rogério Ferreti
• Format: Article
• Language: English
• Subjects: candida; DENTISTRY, ORAL SURGERY & MEDICINE; precursor cell lymphoblastic leukemia-lymphoma; prevalence
• ISSN: 0103-6971; 1981-8637; 1981-8637
• DOI: 10.1590/1981-86372019000183558

ABSTRACT Objective: The aim of this study was to evaluate the prevalence of Candida colonizationon oral cavity of pediatric individuals with acute lymphocytic leukemia (ALL) and its susceptibility/resistance to nystatin and amphotericin B. Methods: This was a cross sectional study with observational, descriptive and analytic approach. Saliva was collected from40 individuals diagnosed with ALL and from40 healthy subjects, as a comparative group, matched by age and gender with ALL group. The mean age for both groups were 8 years-old. The isolation and identification of the Candidaspecies were performed using the CHROMagarCandidaTM and confirmed by polymerase chain reaction. The samples were subjected to antifungal susceptibility by microdilution assay for nystatin and amphotericin B. Salivary alterations and chemotherapy-induced oralmucositis were evaluated using modifiedOral Assessment Guide. Results: The positivity to Candida was higher inALL individuals (32.5%,13/40)than in a comparative group(2.5%, 1/40) (p