Estimation of the cellular antioxidant response to chromium action using ESR method

In the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 microM (nontoxic) and 20 microM (toxic) chromium (VI) concentrations were tested...

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Bibliographic Details
Published in:TheScientificWorld 2004-09, Vol.4, p.785-794
Main Authors: Kartvelishvili, Tamar, Abuladze, Marina, Asatiani, Nino, Akhvlediani, Joseph, Kiziria, Eugene, Asanishvili, Lali, Lejava, Lia, Holman, Hoi-Ying N, Sapojnikova, Nelly
Format: Article
Language:English
Subjects:
antioxidant systems
Antioxidants - metabolism
BASIC BIOLOGICAL SCIENCES
biochemistry
Catalase - antagonists & inhibitors
Catalase - metabolism
cell culture
Cell Extracts
Cell Line
chromium (VI)
Chromium - pharmacology
Electron Spin Resonance Spectroscopy - methods
Epithelial Cells - chemistry
Epithelial Cells - drug effects
Epithelial Cells - enzymology
Epithelial Cells - metabolism
ESR
Glutathione - metabolism
Humans
Sodium Azide - pharmacology
toxicology
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Summary:In the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 microM (nontoxic) and 20 microM (toxic) chromium (VI) concentrations were tested in the model Fenton system with and without catalase-inhibitor sodium azide. The presented approach using the ESR technique along with inhibitors lets us discern cell extract defense capacity connected with the enzymatic activity in viable cells and the catabolic activity in dying cells.
ISSN:1537-744X
2356-6140
1537-744X
DOI:10.1100/tsw.2004.136