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Estimation of the cellular antioxidant response to chromium action using ESR method

In the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 microM (nontoxic) and 20 microM (toxic) chromium (VI) concentrations were tested...

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Published in:	TheScientificWorld 2004-09, Vol.4, p.785-794
Main Authors:	Kartvelishvili, Tamar, Abuladze, Marina, Asatiani, Nino, Akhvlediani, Joseph, Kiziria, Eugene, Asanishvili, Lali, Lejava, Lia, Holman, Hoi-Ying N, Sapojnikova, Nelly
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Language:	English
Subjects:	antioxidant systems Antioxidants - metabolism BASIC BIOLOGICAL SCIENCES biochemistry Catalase - antagonists & inhibitors Catalase - metabolism cell culture Cell Extracts Cell Line chromium (VI) Chromium - pharmacology Electron Spin Resonance Spectroscopy - methods Epithelial Cells - chemistry Epithelial Cells - drug effects Epithelial Cells - enzymology Epithelial Cells - metabolism ESR Glutathione - metabolism Humans Sodium Azide - pharmacology toxicology
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cited_by	cdi_FETCH-LOGICAL-c3906-f6ad2c5529ac1a20d54ec4f896046d2828ce5603d92c7e55f9ab2f780e9837373
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creator	Kartvelishvili, Tamar Abuladze, Marina Asatiani, Nino Akhvlediani, Joseph Kiziria, Eugene Asanishvili, Lali Lejava, Lia Holman, Hoi-Ying N Sapojnikova, Nelly
description	In the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 microM (nontoxic) and 20 microM (toxic) chromium (VI) concentrations were tested in the model Fenton system with and without catalase-inhibitor sodium azide. The presented approach using the ESR technique along with inhibitors lets us discern cell extract defense capacity connected with the enzymatic activity in viable cells and the catabolic activity in dying cells.
doi_str_mv	10.1100/tsw.2004.136
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The presented approach using the ESR technique along with inhibitors lets us discern cell extract defense capacity connected with the enzymatic activity in viable cells and the catabolic activity in dying cells.</description><subject>antioxidant systems</subject><subject>Antioxidants - metabolism</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>biochemistry</subject><subject>Catalase - antagonists & inhibitors</subject><subject>Catalase - metabolism</subject><subject>cell culture</subject><subject>Cell Extracts</subject><subject>Cell Line</subject><subject>chromium (VI)</subject><subject>Chromium - pharmacology</subject><subject>Electron Spin Resonance Spectroscopy - methods</subject><subject>Epithelial Cells - chemistry</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - enzymology</subject><subject>Epithelial Cells - metabolism</subject><subject>ESR</subject><subject>Glutathione - metabolism</subject><subject>Humans</subject><subject>Sodium Azide - pharmacology</subject><subject>toxicology</subject><issn>1537-744X</issn><issn>2356-6140</issn><issn>1537-744X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkd9rFDEQx4MotlbffJbgs3fm925eBClXLRQEq-BbyE6S25TdzZHkqv73zfWKtuRhwsw3n5nMF6G3lKwpJeRjLb_XjBCxplw9Q6dU8m7VCfHr-aP7CXpVyg0hvO-ofIlOWkFoSftTdL0pNc62xrTgFHAdPQY_TfvJZmyXlv4TXYs4-7JLS_G4JgxjTnPcz9jC_bt9icsWb66_49nXMbnX6EWwU_FvHuIZ-nmx-XH-dXX17cvl-eerFXBN1Coo6xhIybQFahlxUngQodeKCOVYz3rwUhHuNIPOSxm0HVjoeuJ1z7t2ztDlkeuSvTG73P6R_5pko7lPpLw1NtcIkzc2qADD0FEahLBOag5tFUOnuSJscLSxPh1Zu_0wewd-qdlOT6BPK0sczTbdGqmlEvowzPsjILWFmgKxehghLYuHaqhiTcab6MNRBDmVkn3414ASc3DTNDfNwU3T3Gzyd4-H-i9-sI_fAZ1UnFc</recordid><startdate>20040902</startdate><enddate>20040902</enddate><creator>Kartvelishvili, Tamar</creator><creator>Abuladze, Marina</creator><creator>Asatiani, Nino</creator><creator>Akhvlediani, Joseph</creator><creator>Kiziria, Eugene</creator><creator>Asanishvili, Lali</creator><creator>Lejava, Lia</creator><creator>Holman, Hoi-Ying N</creator><creator>Sapojnikova, Nelly</creator><general>Hindawi</general><general>TheScientificWorldJOURNAL</general><general>Hindawi Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OIOZB</scope><scope>OTOTI</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20040902</creationdate><title>Estimation of the cellular antioxidant response to chromium action using ESR method</title><author>Kartvelishvili, Tamar ; Abuladze, Marina ; Asatiani, Nino ; Akhvlediani, Joseph ; Kiziria, Eugene ; Asanishvili, Lali ; Lejava, Lia ; Holman, Hoi-Ying N ; Sapojnikova, Nelly</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3906-f6ad2c5529ac1a20d54ec4f896046d2828ce5603d92c7e55f9ab2f780e9837373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>antioxidant systems</topic><topic>Antioxidants - metabolism</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>biochemistry</topic><topic>Catalase - antagonists & inhibitors</topic><topic>Catalase - metabolism</topic><topic>cell culture</topic><topic>Cell Extracts</topic><topic>Cell Line</topic><topic>chromium (VI)</topic><topic>Chromium - pharmacology</topic><topic>Electron Spin Resonance Spectroscopy - methods</topic><topic>Epithelial Cells - chemistry</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - enzymology</topic><topic>Epithelial Cells - metabolism</topic><topic>ESR</topic><topic>Glutathione - metabolism</topic><topic>Humans</topic><topic>Sodium Azide - pharmacology</topic><topic>toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kartvelishvili, Tamar</creatorcontrib><creatorcontrib>Abuladze, Marina</creatorcontrib><creatorcontrib>Asatiani, Nino</creatorcontrib><creatorcontrib>Akhvlediani, Joseph</creatorcontrib><creatorcontrib>Kiziria, Eugene</creatorcontrib><creatorcontrib>Asanishvili, Lali</creatorcontrib><creatorcontrib>Lejava, Lia</creatorcontrib><creatorcontrib>Holman, Hoi-Ying N</creatorcontrib><creatorcontrib>Sapojnikova, Nelly</creatorcontrib><creatorcontrib>Lawrence Berkeley National Lab. 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subjects	antioxidant systems Antioxidants - metabolism BASIC BIOLOGICAL SCIENCES biochemistry Catalase - antagonists & inhibitors Catalase - metabolism cell culture Cell Extracts Cell Line chromium (VI) Chromium - pharmacology Electron Spin Resonance Spectroscopy - methods Epithelial Cells - chemistry Epithelial Cells - drug effects Epithelial Cells - enzymology Epithelial Cells - metabolism ESR Glutathione - metabolism Humans Sodium Azide - pharmacology toxicology
title	Estimation of the cellular antioxidant response to chromium action using ESR method
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