Tumor Necrosis Factor-Alpha Gene Promoter Region Polymorphism and the Risk of Coronary Heart Disease

• Published in: TheScientificWorld 2013-01, Vol.2013 (2013), p.1-5
• Main Authors: Javed, Qamar, Kazmi, Syed Ali Raza, Murtaza, Iram, Liaquat, Afrose, Asifa, Gul Zareen
• Format: Article
• Language: English
• Subjects: Adult; Analysis; Atherosclerosis; Cardiovascular disease; Case-Control Studies; Cholesterol; Coronary Disease - genetics; Coronary heart disease; Female; Genetic polymorphisms; Genetic Predisposition to Disease; Haplotypes; Heart diseases; Humans; Kinases; Male; Middle Aged; Minority & ethnic groups; Necrosis; Pakistan; Pathogenesis; Polymerase chain reaction; Polymorphism; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Risk; Risk factors; Studies; Tumor necrosis factor; Tumor Necrosis Factor-alpha - genetics
• ISSN: 2356-6140; 1537-744X; 1537-744X
• DOI: 10.1155/2013/203492

Tumor necrosis factor-alpha (TNF-α) gene polymorphisms have been implicated in the manifestation of atherosclerosis. Controversy exists regarding the link between the cytokine’s variant genotype and CHD among different ethnic groups. There have been fewer studies on the TNF-α gene −1031T>C and −863C>A polymorphisms in relation to CHD. Therefore, the current study was designed to investigate the association of the TNF-α gene −1031T>C and −863C>A polymorphisms with CHD in a Pakistani population. Methods. Patients with CHD (n=310) and healthy individuals (n=310) were enrolled in this study. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results. A significant difference was observed in the −863C>A polymorphism between patients with CHD and control subjects (PC polymorphism did not show any link with the disease in a Pakistani population.