Immunogenicity assessment of Brucella mellitensis HSP and TF proteins by immunized rabbit serum

Bacteria of the genus Brucella are facultative intracellular pathogens which have developed the capacity to survive and multiply in professional and nonprofessional phagocytes. Due to drawbacks of live attenuated vaccines, much attention has been focused on screening Brucella-protective antigens as...

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Bibliographic Details
Published in:Iranian journal of allergy, asthma, and immunology asthma, and immunology, 2013-06, Vol.12 (2), p.192-194
Main Authors: Ghasemi, Amir, Salari, Mohammad Hossein, Zarnani, Amir Hassan, Pourmand, Mohammad Reza, Ahmadi, Hojat, Shirazi, Mohamad Hassan, Jeddi-Tehrani, Mahmood
Format: Article
Language:English
Subjects:
Animals
Bacteria
Bacterial Proteins - immunology
Bacteriology
Blotting, Western
Brucella
Brucella melitensis
Brucella melitensis - immunology
Brucellosis
Brucellosis - prevention & control
Cloning
E coli
ELISA
Enzyme-Linked Immunosorbent Assay
Heat shock proteins
Heat-Shock Proteins - immunology
Immunization
Immunogenicity
Medicin och hälsovetenskap
Protein Expression
Purification
Rabbits
Vaccines - immunology
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Summary:Bacteria of the genus Brucella are facultative intracellular pathogens which have developed the capacity to survive and multiply in professional and nonprofessional phagocytes. Due to drawbacks of live attenuated vaccines, much attention has been focused on screening Brucella-protective antigens as subunit vaccine candidates. In order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, the authors cloned, expressed and purified Heat Shock Protein (HSP) and Trigger Factor (TF) from Brucella melitensis. These recombinant antigens were then evaluated by serum from a B. mellitensis-vaccinated rabbit using ELISA and Western blot. Their results showed that the immunized rabbit serum reacted with recombinant HSP and TF in ELISA and Western blot. These results may suggest that B.melitensis rTF and rHSP may serve as candidate subunit vaccine components for protection against the infection. Brucella spp. are Gram-negative and facultative intracellular bacteria which cause brucellosis, a worldwide zoonotic disease causing abortion in domestic animals and Malta fever in humans.
ISSN:1735-1502
1735-5249