The influence of a magnetic field on photon beam radiotherapy in a normal human TK6 lymphoblastoid cell line

• Published in: Radiation oncology (London, England) England), 2019-01, Vol.14 (1), p.11-11, Article 11
• Main Authors: Yudhistiara, B, Zwicker, F, Weber, K J, Huber, P E, Ruehle, A, Brons, S, Haering, P, Debus, J, Hauswald, S H
• Format: Article
• Language: English
• Subjects: Apoptosis; Cancer; Cell cycle; Cell lines; Cells (Biology); Cooperation; Deoxyribonucleic acid; Dependence; Diagnostic imaging; DNA; Experiments; Flow cytometry; Genetic research; In-vitro experiment; Ionizing radiation; Irradiation; Magnetic fields; Magnetic resonance imaging; Medical imaging; Medical research; MR Linac; MRI guided radiotherapy; NMR; Normal human cells; Nuclear magnetic resonance; Oncology; Parameter sensitivity; Photon beams; Photons; Physics; Radiation (Physics); Radiation dosage; Radiation effects; Radiation therapy; Radiotherapy; Research centers; Risk factors; Survival; TK6 human lymphoblastoid cells
• ISSN: 1748-717X; 1748-717X
• DOI: 10.1186/s13014-019-1212-5

The implementation of magnetic resonance imaging (MRI) guided radiotherapy (RT) continues to increase. Very limited in-vitro data on the interaction of ionizing radiation and magnetic fields (MF) have been published. In these experiments we focused on the radiation response in a MF of the TK6 human lymphoblastoid cells which are known to be highly radiosensitive due to efficient radiation-induced apoptosis. Clonogenicity was determined 12-14 days after irradiation with 1-4 Gy 6 MV photons with or without a 1.0 Tesla MF. Furthermore, alterations in cell cycle distribution and rates of radiation induced apoptosis (FACS analysis of cells with sub-G1 DNA content) were analyzed. Clonogenic survival showed an exponential dose-dependence, and the radiation sensitivity parameter (α = 1.57/Gy) was in accordance with earlier reports. Upon comparing the clonogenic survival between the two groups, identical results within error bars were obtained. The survival fractions at 2 Gy were 9% (without MF) and 8.5% (with MF), respectively. A 1.0 Tesla MF does not affect the clonogenicity of TK6 cells irradiated with 1-4 Gy 6MV photons. This supports the use of MRI guided RT, however ongoing research on the interaction of MF and radiotherapy is warranted.