Eimeria zuernii (Eimeriidae: Coccidia): mitochondrial genome and genetic diversity in the Chinese yak

• Published in: Parasites & vectors 2023-09, Vol.16 (1), p.1-312, Article 312
• Main Authors: Zhou, Xuan, Wang, Zhao, Zhu, Pengchen, Gu, Xiaobin, He, Ran, Xu, Jing, Jing, Bo, Wang, Lidan, Chen, Shun, Xie, Yue
• Format: Article
• Language: English
• Subjects: Analysis; Biological diversity; Biological evolution; Biology; Breeding; Cattle; Causes of; Coccidia; Coccidiosis; Control; Cytochrome; Cytochrome b; Cytochrome-c oxidase; Cytochromes; Diseases; Distribution; Eimeria; Eimeria zuernii; Eimeriidae; Evolution; Genes; Genetic aspects; Genetic diversity; Genetic relationships; Genetic research; Genetic structure; Genetic variation; Genomes; Genomics; Haplotypes; Mitochondrial DNA; MtDNA; Natural history; Nucleotides; Oxidases; Parasites; Parasitological research; Phylogenetics; Phylogeny; Population; Population genetics; Population structure; Populations; Protozoa; Ribosomal DNA; Yak coccidiosis; Yaks
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-023-05925-8

Coccidiosis caused by Eimeria zuernii (Eimeriidae: Coccidia) represents a significant economic threat to the bovine industry. Understanding the evolutionary and genetic biology of E. zuernii can assist in new interaction developments for the prevention and control of this protozoosis. We defined the evolutionary and genetic characteristics of E. zuernii by sequencing the complete mitogenome and analyzing the genetic diversity and population structure of 51 isolates collected from eight yak breeding parks in China. The 6176-bp mitogenome of E. zuernii was linear and encoded typical mitochondrial contents of apicomplexan parasites, including three protein-coding genes [PCGs; cytochrome c oxidase subunits I and III (cox1 and cox3), and cytochrome b (cytb)], seven fragmented small subunit (SSU) and 12 fragmented large subunit (LSU) rRNAs. Genome-wide comparative and evolutionary analyses showed cytb and cox3 to be the most and least conserved Eimeria PCGs, respectively, and placed E. zuernii more closely related to Eimeria mephitidis than other Eimeria species. Furthermore, cox1-based genetic structure defined 24 haplotypes of E. zuernii with high haplotype diversities and low nucleotide diversities across eight geographic populations, supporting a low genetic structure and rapid evolutionary rate as well as a previous expansion event among E. zuernii populations. To our knowledge, this is the first study presenting the phylogeny, genetic diversity, and population structure of the yak E. zuernii, and such information, together with its mitogenomic data, should contribute to a better understanding of the genetic and evolutionary biological studies of apicomplexan parasites in bovines.