Glucose increases interleukin-12 gene expression and production in stimulated peripheral blood mononuclear cells of type 2 diabetes patients

Lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) of type 2 diabetes patients produce more interleukin (IL)-12 under glucose treatment. The aim of this study was to determine whether increased IL-12 response in hyperglycemic LPS-stimulated PBMCs is due to increased gene...

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Bibliographic Details
Published in:Biomedical journal 2014-09, Vol.37 (5), p.293-297
Main Authors: Chu, Chien-Ming, Kuo, Sheng-Fong, Hua, Chung-Ching, Wu, Shao-Yun, Chuang, Duen-Yau, Wu, Huang-Pin
Format: Article
Language:English
Subjects:
Adult
Aged
Atherosclerosis
Cytokines
Deoxyribonucleic acid
Diabetes
Diabetes Mellitus, Type 2 - metabolism
DNA
Female
Gene expression
Gene Expression Regulation - drug effects
Glucose - metabolism
Humans
Hyperglycemia
Insulin
interleukin-12
Interleukin-12 - genetics
Interleukin-12 - metabolism
Leukocytes, Mononuclear - metabolism
Lipopolysaccharides - pharmacology
Male
Middle Aged
osmolarity
peripheral blood mononuclear cells
Proteins
Sepsis
Statistical analysis
Studies
Tumor necrosis factor-TNF
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Summary:Lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) of type 2 diabetes patients produce more interleukin (IL)-12 under glucose treatment. The aim of this study was to determine whether increased IL-12 response in hyperglycemic LPS-stimulated PBMCs is due to increased gene expression or osmolarity. LPS-stimulated PBMCs of 13 type 2 diabetes patients and 8 healthy controls were used for culture in the presence or absence of glucose or mannitol for 24 h. The IL-12 gene expressions of PBMCs and IL-12 protein levels in supernatants were evaluated. After 24 h, the stimulated PBMCs of diabetes patients expressed more IL-12 mRNA and produced more IL-12 protein following glucose treatment than those without glucose treatment and with mannitol treatment. Stimulated PBMCs of controls did not express more IL-12 mRNA and produce more IL-12 protein following glucose treatment than those without glucose treatment and with mannitol treatment. Glucose increases the IL-12 production in stimulated PBMCs of diabetes patients through increased IL-12 gene expression.
ISSN:2319-4170
2320-2890
DOI:10.4103/2319-4170.132887