A protocol for measuring the sexual receptivity of female Drosophila

Female receptivity in mating is crucial for successful copulation, but protocols for quantifying female behaviors reflecting receptivity are scarce compared to the analysis of male behaviors. Here, we present a protocol for assessing the sexual receptivity of female Drosophila that considers behavio...

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Bibliographic Details
Published in:STAR protocols 2023-12, Vol.4 (4), p.102563-102563, Article 102563
Main Authors: Yang, Yan Tong, Hu, Shao Wei, Zhu, Yan
Format: Article
Language:English
Subjects:
Animals
Behavior
Courtship
Drosophila
Drosophila Proteins
Female
Genetics
Male
Model Organisms
Protocol
Sexual Behavior, Animal
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Summary:Female receptivity in mating is crucial for successful copulation, but protocols for quantifying female behaviors reflecting receptivity are scarce compared to the analysis of male behaviors. Here, we present a protocol for assessing the sexual receptivity of female Drosophila that considers behaviors from both sexes. We describe steps for preparing and loading flies into a courtship chamber, video recording the behaviors of the pairs, and analyzing their behavioral displays. This protocol includes behavior recognition criteria suitable for typical laboratory settings. For complete details on the use and execution of this protocol, please refer to Yang et al. (2023).1 [Display omitted] •A comprehensive protocol for quantifying Drosophila female receptivity•Assessment of key parameters indicative of female receptivity•Analyzing interplays between males and females during courtship Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Female receptivity in mating is crucial for successful copulation, but protocols for quantifying female behaviors reflecting receptivity are scarce compared to the analysis of male behaviors. Here, we present a protocol for assessing the sexual receptivity of female Drosophila that considers behaviors from both sexes. We describe steps for preparing and loading flies into a courtship chamber, video recording the behaviors of the pairs, and analyzing their behavioral displays. This protocol includes behavior recognition criteria suitable for typical laboratory settings.
ISSN:2666-1667
2666-1667
DOI:10.1016/j.xpro.2023.102563