1,2-3H-Cholesterol as a tracer in studies of human cholesterol metabolism

1,2-3H-Cholesterol was fed to a subject together with 4-14C-cholesterol at a known 3H/14C ratio. The ratio was satisfactorily preserved in cholesterol recovered from plasma, red cells, and bile, and in bile acids of bile.Isotopic fractionation was seen during thin-layer chromatographic isolation of...

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Bibliographic Details
Published in:Journal of lipid research 1967-07, Vol.8 (4), p.406-408
Main Authors: Wood, Peter D.S., Myers, Daphne, Lee, Yuen-Ling, Shioda, Ryuzo, Kinsell, Laurance W.
Format: Article
Language:English
Subjects:
1,2-3H-cholesterol
4-14C-cholesterol
cholesterol
isotope effect
plasma
thin-layer chromatography
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Summary:1,2-3H-Cholesterol was fed to a subject together with 4-14C-cholesterol at a known 3H/14C ratio. The ratio was satisfactorily preserved in cholesterol recovered from plasma, red cells, and bile, and in bile acids of bile.Isotopic fractionation was seen during thin-layer chromatographic isolation of cholesterol. In work with 1,2-3H-cholesterol or its metabolites care should be taken to recover chromatographic bands in their entirety.
ISSN:0022-2275
DOI:10.1016/S0022-2275(20)39573-0