Suppression of Transferrin Expression Enhances the Susceptibility of Plutella xylostella to Isaria cicadae

Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a ( ) from was identified and characterized. The consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2020-05, Vol.11 (5), p.281
Main Authors: Xu, Huihui, Hao, Zhongping, Wang, Lifang, Li, Shuangjiao, Guo, Yuruo, Dang, Xiangli
Format: Article
Language:English
Subjects:
Amino acids
Binding sites
Biopesticides
Cloning
Deoxyribonucleic acid
development
DNA
expression patterns
Fungi
Gene expression
Hemocytes
immunity
Immunology
Insecticides
Insects
Iron
Isaria cicadae
Larvae
Levels
Metabolism
Microorganisms
Molecular weight
Nodulation
Pathogens
Peptides
Pest control
Plutella xylostella
Proteins
Pupation
RNA-mediated interference
Transcription
Transferrin
Transferrins
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Summary:Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a ( ) from was identified and characterized. The consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae. transcript level was highest in fat bodies among various tissues. The transcript levels increased significantly after the stimulation of pathogens. A decrease in expression via RNA interference enhanced the susceptibility of to the fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in immunity against fungal infection and insect development.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects11050281