An in vitro model system based on calcium- and phosphate ion-induced hMSC spheroid mineralization

A challenge in regenerative medicine is creating the three-dimensional organic and inorganic in vitro microenvironment of bone, which would allow the study of musculoskeletal disorders and the generation of building blocks for bone regeneration. This study presents a microwell-based platform for cre...

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Bibliographic Details
Published in:Materials today bio 2023-12, Vol.23, p.100844-100844, Article 100844
Main Authors: Vermeulen, Steven, Knoops, Kèvin, Duimel, Hans, Parvizifard, Maryam, van Beurden, Denis, López-Iglesias, Carmen, Giselbrecht, Stefan, Truckenmüller, Roman, Habibović, Pamela, Tahmasebi Birgani, Zeinab
Format: Article
Language:English
Subjects:
Bone
Calcium
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Mesenchymal stromal cells
Mineralization
Phosphate
Regenerative medicine
Spheroids
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Summary:A challenge in regenerative medicine is creating the three-dimensional organic and inorganic in vitro microenvironment of bone, which would allow the study of musculoskeletal disorders and the generation of building blocks for bone regeneration. This study presents a microwell-based platform for creating spheroids of human mesenchymal stromal cells, which are then mineralized using ionic calcium and phosphate supplementation. The resulting mineralized spheroids promote an osteogenic gene expression profile through the influence of the spheroids’ biophysical environment and inorganic signaling and require less calcium or phosphate to achieve mineralization compared to a monolayer culture. We found that mineralized spheroids represent an in vitro model for studying small molecule perturbations and extracellular mediated calcification. Furthermore, we demonstrate that understanding pathway signaling elicited by the spheroid environment allows mimicking these pathways in traditional monolayer culture, enabling similar rapid mineralization events. In sum, this study demonstrates the rapid generation and employment of a mineralized cell model system for regenerative medicine applications. [Display omitted]
ISSN:2590-0064
2590-0064
DOI:10.1016/j.mtbio.2023.100844