Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life

DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quali...

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Bibliographic Details
Published in:Metabarcoding and Metagenomics 2021-02, Vol.5, p.17-33
Main Authors: Rimet, Frédéric, Aylagas, Eva, Borja, Ángel, Bouchez, Agnès, Canino, Alexis, Chauvin, Christian, Chonova, Teofana, Ciampor Jr, Fedor, Costa, Filipe O., Ferrari, Benoit J. D., Gastineau, Romain, Goulon, Chloé, Gugger, Muriel, Holzmann, Maria, Jahn, Regine, Kahlert, Maria, Kusber, Wolf-Henning, Laplace-Treyture, Christophe, Leese, Florian, Leliaert, Frederik, Mann, David G., Marchand, Frédéric, Méléder, Vona, Pawlowski, Jan, Rasconi, Serena, Rivera, Sinziana, Rougerie, Rodolphe, Schweizer, Magali, Trobajo, Rosa, Vasselon, Valentin, Vivien, Régis, Weigand, Alexander, Witkowski, Andrzej, Zimmermann, Jonas, Ekrem, Torbjørn
Format: Article
Language:English
Subjects:
Aquatic organisms
Barcode
Ciências Biológicas
Ciências Naturais
DNA
Engenharia do Ambiente
Engenharia e Tecnologia
Genetics
Genetik
Life Sciences
Metadata
Quality
Reference library
Traceability
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Description
Summary:DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data
ISSN:2534-9708
2534-9708
DOI:10.3897/mbmg.5.58056