In Vitro Evaluation of Biological Activities and Phytochemical Analysis of Different Solvent Extracts of Punica granatum L. (Pomegranate) Peels

Antimicrobial resistance is a public health concern resulting in high rates of morbidity and mortality worldwide. Furthermore, a high incidence of food poisoning diseases besides harmful implications of applying synthetic food additives in food preservation necessitates fabrication of safe food pres...

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Bibliographic Details
Published in:Plants (Basel) 2021-12, Vol.10 (12), p.2742
Main Authors: Yassin, Mohamed Taha, Mostafa, Ashraf Abdel-Fattah, Al Askar, Abdulaziz Abdulrahman
Format: Article
Language:English
Subjects:
antibacterial
Antimicrobial agents
Antimicrobial resistance
Antioxidants
antiradical
Bacteria
Breast cancer
Cancer
Cancer therapies
carcinopreventive
Cardiovascular diseases
Cell division
Chemotherapy
Cytotoxicity
Diabetes mellitus
Drug resistance
E coli
Efficiency
Fabrication
Fibroblasts
Flavonoids
Food additives
Food contamination
Food contamination & poisoning
Food poisoning
Food preservation
food spoilage
Fractions
Free radicals
gas chromatography-mass spectrometry
Hydroxymethylfurfural
Minimum inhibitory concentration
Morbidity
Mortality
Natural products
Pathogens
Phytochemicals
Plant extracts
Polyphenols
pomegranate
Preservatives
Public health
Punica granatum
Recording
Scavenging
Selectivity
Solvents
Staphylococcus aureus
Staphylococcus infections
Synthetic food
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Summary:Antimicrobial resistance is a public health concern resulting in high rates of morbidity and mortality worldwide. Furthermore, a high incidence of food poisoning diseases besides harmful implications of applying synthetic food additives in food preservation necessitates fabrication of safe food preservatives. Additionally, damaging effects of free radicals on human health has been reported to be involved in the incidence of serious diseases, including cancer, diabetes and cardiovascular diseases; hence, finding safe sources of antioxidants is vital. Therefore, the present study was carried out to assess the antibacterial, antiradical and carcinopreventive efficacy of different solvent extracts of pomegranate peels. Agar disk diffusion assay revealed that , MRSA, and were highly susceptible to methanolic fraction of L. peels recording inhibition zones of 23.7, 21.8, 15.6 and 14.7 mm respectively. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the methanolic fraction of L. peels against were 0.125 and 0.250 mg/mL, respectively. In addition, the pomegranate acetonic and methanolic fractions revealed an impressive antiradical efficiency against DPPH (2,2-diphenyl-1-picrylhydrazyl) radical recording radical scavenging activity percentages of 86.9 and 79.4%, respectively. In this regard, the acetonic fraction of pomegranate peels revealed the highest anti-proliferative efficiency after 48 h incubation against MCF7 cancer cells recording IC of 8.15 µg/mL, while the methanolic extract was highly selective against transformed cancer cells compared to normal cell line recording selectivity index of 5.93. GC-MS results demonstrated that 5-Hydroxymethylfurfural was the main active component of methanolic and acetonic extracts of pomegranate peels recording relative percentages of 37.55 and 28.84% respectively. The study recommends application of pomegranate peel extracts in the biofabrication of safe food preservatives, antioxidants and carcinopreventive agents.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10122742