Uncovering the Role of PhzC as DAHP Synthase in Shikimate Pathway of Pseudomonas chlororaphis HT66

DAHP synthase catalyzes the first step in the shikimate pathway, deriving the biosynthesis of aromatic amino acids (Trp, Phe and Tyr), phenazine-1-carboxamide, folic acid, and ubiquinone in . In this study, we identified and characterized one DAHP synthase encoding gene , which differs from the repo...

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Bibliographic Details
Published in:Biology (Basel, Switzerland) Switzerland), 2022-01, Vol.11 (1), p.86
Main Authors: Wang, Songwei, Liu, Dongliang, Bilal, Muhammad, Wang, Wei, Zhang, Xuehong
Format: Article
Language:English
Subjects:
Allosteric properties
Amino acids
Binding sites
Biosynthesis
Cloning
Complementation
E coli
Enzymes
Feedback inhibition
Fermentation
Folic acid
Genes
Genetic engineering
Glycerol
Metabolism
Metabolites
Phenazine
phenazine-1-carboxamide
PhzC
Plasmids
Pseudomonas chlororaphis
shikimate pathway
Ubiquinone
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Summary:DAHP synthase catalyzes the first step in the shikimate pathway, deriving the biosynthesis of aromatic amino acids (Trp, Phe and Tyr), phenazine-1-carboxamide, folic acid, and ubiquinone in . In this study, we identified and characterized one DAHP synthase encoding gene , which differs from the reported DAHP synthase encoding genes , and in . . PhzC accounts for approximately 90% of the total DAHP synthase activities in . HT66 and plays the most critical role in four DAHP synthases in the shikimate pathway. Inactivation of resulted in the reduction of PCN production by more than 90%, while the absence of genes , and reduced PCN yield by less than 15%, and the production of PCN was restored after the complementation of gene . Moreover, the results showed that in . HT66 is not sensitive to feedback inhibition. This study demonstrated that gene is essential for PCN biosynthesis. The expression level of both and genes are not inhibited in feedback by PCN production due to the absence of a loop region required for allosteric control reaction. This study highlighted the importance of PhzC and applying . for shikimate pathway-derived high-value biological production.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology11010086