Passive Immunotherapy of Cynomolgus Monkeys with Anti-Rotavirus IgY

Immunoglobulins Y (IgY) purified from egg yolks of hens represents an attractive, cost-effective alternative for the development of new diagnostic and therapeutic platforms. In this study, we evaluated the therapeutic efficacy of rotavirus-specific IgY in a cynomolgus monkey ( ) model. Animals were...

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Bibliographic Details
Published in:Pharmaceutics 2024-08, Vol.16 (9), p.1149
Main Authors: Bentes, Gentil Arthur, Guimarães, Juliana Rodrigues, Volotão, Eduardo de Mello, Lanzarini, Natália Maria, da Silva, Alexandre Dos Santos, Gardinali, Noemi Rovaris, Marchevsky, Renato Sergio, Leite, José Paulo Gagliardi, de Oliveira, Jaqueline Mendes, Pinto, Marcelo Alves
Format: Article
Language:English
Subjects:
Antibodies
cynomolgus monkeys
Diarrhea
Electrolytes
Gastroenteritis
Health aspects
Hematology
human rotavirus Group A
Humidity
immunoglobulin Y
Immunotherapy
Infections
Infectious diseases
Laboratory animals
Monkeys & apes
passive immunotherapy
Quarantine
Rotavirus
Serology
Small intestine
Viruses
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Summary:Immunoglobulins Y (IgY) purified from egg yolks of hens represents an attractive, cost-effective alternative for the development of new diagnostic and therapeutic platforms. In this study, we evaluated the therapeutic efficacy of rotavirus-specific IgY in a cynomolgus monkey ( ) model. Animals were experimentally infected with human rotavirus Group A (RVA), the most common cause of severe acute diarrhoea among young children worldwide. Animals were administered human RVA (3.1 × 10 FFU/mL) by oral gavage, challenged with 2.5 mg of anti-RVA IgY orally, and monitored for five days according to clinical, haematological and biochemical parameters; serum electrolyte levels; viral shedding; and histopathological changes. Immunotherapy with anti-RVA IgY had a protective effect against severe rotavirus-induced enteritis in four of the ten treated monkeys, as evidenced by histopathological findings. Although only one animal had diarrhoea, all but one exhibited virus shedding regardless of the treatment.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics16091149