Passive Immunotherapy of Cynomolgus Monkeys with Anti-Rotavirus IgY

Immunoglobulins Y (IgY) purified from egg yolks of hens represents an attractive, cost-effective alternative for the development of new diagnostic and therapeutic platforms. In this study, we evaluated the therapeutic efficacy of rotavirus-specific IgY in a cynomolgus monkey ( ) model. Animals were...

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Published in:Pharmaceutics 2024-08, Vol.16 (9), p.1149
Main Authors: Bentes, Gentil Arthur, Guimarães, Juliana Rodrigues, Volotão, Eduardo de Mello, Lanzarini, Natália Maria, da Silva, Alexandre Dos Santos, Gardinali, Noemi Rovaris, Marchevsky, Renato Sergio, Leite, José Paulo Gagliardi, de Oliveira, Jaqueline Mendes, Pinto, Marcelo Alves
Format: Article
Language:English
Subjects:
Antibodies
cynomolgus monkeys
Diarrhea
Electrolytes
Gastroenteritis
Health aspects
Hematology
human rotavirus Group A
Humidity
immunoglobulin Y
Immunotherapy
Infections
Infectious diseases
Laboratory animals
Monkeys & apes
passive immunotherapy
Quarantine
Rotavirus
Serology
Small intestine
Viruses
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container_end_page
container_issue 9
container_start_page 1149
container_title Pharmaceutics
container_volume 16
creator Bentes, Gentil Arthur
Guimarães, Juliana Rodrigues
Volotão, Eduardo de Mello
Lanzarini, Natália Maria
da Silva, Alexandre Dos Santos
Gardinali, Noemi Rovaris
Marchevsky, Renato Sergio
Leite, José Paulo Gagliardi
de Oliveira, Jaqueline Mendes
Pinto, Marcelo Alves
description Immunoglobulins Y (IgY) purified from egg yolks of hens represents an attractive, cost-effective alternative for the development of new diagnostic and therapeutic platforms. In this study, we evaluated the therapeutic efficacy of rotavirus-specific IgY in a cynomolgus monkey ( ) model. Animals were experimentally infected with human rotavirus Group A (RVA), the most common cause of severe acute diarrhoea among young children worldwide. Animals were administered human RVA (3.1 × 10 FFU/mL) by oral gavage, challenged with 2.5 mg of anti-RVA IgY orally, and monitored for five days according to clinical, haematological and biochemical parameters; serum electrolyte levels; viral shedding; and histopathological changes. Immunotherapy with anti-RVA IgY had a protective effect against severe rotavirus-induced enteritis in four of the ten treated monkeys, as evidenced by histopathological findings. Although only one animal had diarrhoea, all but one exhibited virus shedding regardless of the treatment.
doi_str_mv 10.3390/pharmaceutics16091149
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subjects Antibodies
cynomolgus monkeys
Diarrhea
Electrolytes
Gastroenteritis
Health aspects
Hematology
human rotavirus Group A
Humidity
immunoglobulin Y
Immunotherapy
Infections
Infectious diseases
Laboratory animals
Monkeys & apes
passive immunotherapy
Quarantine
Rotavirus
Serology
Small intestine
Viruses
title Passive Immunotherapy of Cynomolgus Monkeys with Anti-Rotavirus IgY
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