Exploring the Potential Mechanism of Action of Piperine against Candida albicans and Targeting Its Virulence Factors

Plant-derived compounds have proven to be a source of inspiration for new drugs. In this study, piperine isolated from the fruits of showed anti- activity. Furthermore, the mechanisms of action of piperine and its impact on virulence factors in , which have not been comprehensively understood, were...

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Published in:Biomolecules (Basel, Switzerland) Switzerland), 2023-11, Vol.13 (12), p.1729
Main Authors: Bravo-Chaucanés, Claudia Patricia, Chitiva, Luis Carlos, Vargas-Casanova, Yerly, Diaz-Santoyo, Valentina, Hernández, Andrea Ximena, Costa, Geison M, Parra-Giraldo, Claudia Marcela
Format: Article
Language:English
Subjects:
antifungal activity
Antifungal agents
Antifungal Agents - metabolism
Antifungal Agents - pharmacology
Biofilms
Candida albicans
Cell cycle
Cell death
Cell membranes
Chromatography
Cytotoxicity
Drug resistance
Fluconazole
Fluconazole - pharmacology
Infections
Iodine
mechanism of action
Membrane permeability
Membrane potential
Morphology
NMR
Nuclear magnetic resonance
Pathogens
Piperine
Plants
Reactive oxygen species
Rhodamine
Scanning electron microscopy
Solvents
Virulence
Virulence factors
Virulence Factors - metabolism
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Summary:Plant-derived compounds have proven to be a source of inspiration for new drugs. In this study, piperine isolated from the fruits of showed anti- activity. Furthermore, the mechanisms of action of piperine and its impact on virulence factors in , which have not been comprehensively understood, were also assessed. Initially, piperine suppressed the hyphal transition in both liquid and solid media, hindered biofilm formation, and resulted in observable cell distortions in scanning electron microscope (SEM) samples, for both fluconazole-sensitive and fluconazole-resistant strains. Additionally, the morphogenetic switches triggered by piperine were found to rely on the activity of mutant strains. Secondly, piperine treatment increased cell membrane permeability and disrupted mitochondrial membrane potential, as evidenced by propidium iodine and Rhodamine 123 staining, respectively. Moreover, it induced the accumulation of intracellular reactive oxygen species in . Synergy was obtained between the piperine and the fluconazole against the fluconazole-sensitive strain. Interestingly, there were no hemolytic effects of piperine, and it resulted in reduced cytotoxicity on fibroblast cells at low concentrations. The results suggest that piperine could have a dual mode of action inhibiting virulence factors and modulating cellular processes, leading to cell death in .
ISSN:2218-273X
2218-273X
DOI:10.3390/biom13121729