Pulsed high-dose dexamethasone modulates Th1-/Th2-chemokine imbalance in immune thrombocytopenia

Chemokines and chemokine receptors play important roles in autoimmune diseases; however, their role in immune thrombocytopenia (ITP) is unclear. High-dose dexamethasone (HD-DXM) may become a first-line therapy for adult patients with ITP, but the effect of HD-DXM on chemokines in ITP patients is unk...

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Bibliographic Details
Published in:Journal of translational medicine 2016-10, Vol.14 (1), p.301-301, Article 301
Main Authors: Liu, Zongtang, Wang, Meiying, Zhou, Shufen, Ma, Ji, Shi, Yan, Peng, Jun, Hou, Ming, Guo, Chengshan
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Care and treatment
Chemokine
Chemokine receptor
Chemokines
Chemokines - blood
Chemokines - genetics
Chemokines - metabolism
Child
Dexamethasone
Dexamethasone - pharmacology
Dexamethasone - therapeutic use
Dosage and administration
Dose-Response Relationship, Drug
Female
Gene expression
Health aspects
High-dose dexamethasone
Humans
Immune thrombocytopenia
Male
Middle Aged
Purpura, Thrombocytopenic, Idiopathic - blood
Purpura, Thrombocytopenic, Idiopathic - drug therapy
Purpura, Thrombocytopenic, Idiopathic - genetics
Receptors, Chemokine - genetics
Receptors, Chemokine - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Th1 Cells - drug effects
Th1 Cells - immunology
Th1 polarization
Th2 Cells - drug effects
Th2 Cells - immunology
Thrombocytopenia
Young Adult
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Summary:Chemokines and chemokine receptors play important roles in autoimmune diseases; however, their role in immune thrombocytopenia (ITP) is unclear. High-dose dexamethasone (HD-DXM) may become a first-line therapy for adult patients with ITP, but the effect of HD-DXM on chemokines in ITP patients is unknown. Our aim was to investigate the mechanism of pulsed HD-DXM for management of ITP, specifically regarding the chemokine pathways. Th1-/Th2-associated chemokine and chemokine receptor profiles in ITP patients before and after pulsed HD-DXM was studied. Plasma levels of CCL5 and CXCL11 (Th1-associated) and of CCL11 (Th2-associated) were determined by ELISA. Gene expression of these three chemokines and their corresponding receptors CCR5, CXCR3, and CCR3, in peripheral blood mononuclear cells (PBMCs) was determined by quantitative RT-PCR. Thirty-three of the thirty-eight ITP patients responded effectively to HD-DXM (oral, 40 mg/day, 4 days). In ITP patients, plasma CXCL11 levels increased, while CCL11 and CCL5 decreased compared to controls (P 
ISSN:1479-5876
1479-5876
DOI:10.1186/s12967-016-1064-9