Cryo-EM structure supports a role of AQP7 as a junction protein

• Published in: Nature communications 2023-02, Vol.14 (1), p.600-12, Article 600
• Main Authors: Huang, Peng, Venskutonytė, Raminta, Prasad, Rashmi B., Ardalani, Hamidreza, de Maré, Sofia W., Fan, Xiao, Li, Ping, Spégel, Peter, Yan, Nieng, Gourdon, Pontus, Artner, Isabella, Lindkvist-Petersson, Karin
• Format: Article
• Language: English
• Subjects: 101/28; 101/58; 14; 14/19; 38; 38/91; 631/45/535/1258/1259; 631/57/2265; 692/699/2743/137/773; 82/1; Aquaglyceroporins; Aquaporins - metabolism; Biologi; Biological Sciences; Cell Biology; Cellbiologi; Cryoelectron Microscopy; Gas chromatography; Glycerol; Glycerol - metabolism; Humanities and Social Sciences; Humans; Islets of Langerhans - metabolism; Leucine; Mass spectrometry; Mass spectroscopy; Medical and Health Sciences; Medical Biotechnology; Medicin och hälsovetenskap; Medicinsk bioteknologi; Membranes; Metabolism; Monomers; multidisciplinary; Natural Sciences; Naturvetenskap; Proteins; Science; Science (multidisciplinary); Supports
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-36272-y

Aquaglyceroporin 7 (AQP7) facilitates glycerol flux across the plasma membrane with a critical physiological role linked to metabolism, obesity, and associated diseases. Here, we present the single-particle cryo-EM structure of AQP7 determined at 2.55 Å resolution adopting two adhering tetramers, stabilized by extracellularly exposed loops, in a configuration like that of the well-characterized interaction of AQP0 tetramers. The central pore, in-between the four monomers, displays well-defined densities restricted by two leucine filters. Gas chromatography mass spectrometry (GC/MS) results show that the AQP7 sample contains glycerol 3-phosphate (Gro3P), which is compatible with the identified features in the central pore. AQP7 is shown to be highly expressed in human pancreatic α- and β- cells suggesting that the identified AQP7 octamer assembly, in addition to its function as glycerol channel, may serve as junction proteins within the endocrine pancreas. Glycerol flux across the plasma membrane is critical to metabolism and linked to disease. Here, authors present the cryo-EM structure of the glycerol channel AQP7 composed of two adhering tetramers and displaying well-defined densities in the central pore.