An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries

Indium phosphide quantum dots (QDs) passivated with zinc sulphide in a core/shell architecture (InP/ZnS) with different surface chemistries were introduced to RAW 264.7 murine "macrophage-like" cells to understand their potential toxicities. The InP/ZnS quantum dots were conjugated with an...

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Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2019-01, Vol.9 (2), p.135
Main Authors: Ayupova, Deanna, Dobhal, Garima, Laufersky, Geoffry, Nann, Thomas, Goreham, Renee V
Format: Article
Language:English
Subjects:
aptamer
bioimaging
cytotoxicity
photoluminescence
quantum dots
quantum yields
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Summary:Indium phosphide quantum dots (QDs) passivated with zinc sulphide in a core/shell architecture (InP/ZnS) with different surface chemistries were introduced to RAW 264.7 murine "macrophage-like" cells to understand their potential toxicities. The InP/ZnS quantum dots were conjugated with an oligonucleotide, a carboxylic acid, or an amino-polyethylene glycol ligand, and cell viability and cell proliferation were investigated via a metabolic assay. Membrane integrity was measured through the production of lactate dehydrogenase. Fluorescence microscopy showed cellular uptake. All quantum dots exhibited cytotoxic behaviour less than that observed from cadmium- or lead-based quantum dots; however, this behaviour was sensitive to the ligands used. In particular, the amino-polyethylene glycol conjugated quantum dots proved to possess the highest cytotoxicity examined here. This provides quantitative evidence that aqueous InP/ZnS quantum dots can offer a safer alternative for bioimaging or in therapeutic applications.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano9020135