Comparative analysis of a BAC contig of porcine chromosome 13q31-q32 and human chromosome 3q21-q22

The gene(s) encoding the ETEC F4ab/ac receptors, involved in neonatal diarrhoea in pigs (a disease not yet described in humans), is located close to the TF locus on Sscr13. In order to reveal and characterize possible candidate genes encoding these receptors, a porcine physical map of the TF region...

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Bibliographic Details
Published in:BMC genomics 2005-09, Vol.6 (1), p.133-133, Article 133
Main Authors: Van Poucke, Mario, Bourry, David, Piumi, François, Mattheeuws, Marc, Van Zeveren, Alex, Chardon, Patrick, Peelman, Luc J
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Chromosome Mapping
Chromosome Walking
Chromosomes, Artificial, Bacterial
Chromosomes, Human, Pair 3
Contig Mapping - methods
DNA Primers - chemistry
Expressed Sequence Tags
Genetic Linkage
Genome, Human
Humans
Life Sciences
Nucleic Acid Hybridization
Physical Chromosome Mapping
Radiation Hybrid Mapping
Species Specificity
Sus scrofa
Swine
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Summary:The gene(s) encoding the ETEC F4ab/ac receptors, involved in neonatal diarrhoea in pigs (a disease not yet described in humans), is located close to the TF locus on Sscr13. In order to reveal and characterize possible candidate genes encoding these receptors, a porcine physical map of the TF region is indispensable. A contig of 33 BAC clones, covering approximately 1.35 Mb surrounding the TF locus on Sscr13q31-q32, was built by chromosome walking. A total of 22,552 bp from the BAC contig were sequenced and compared with database sequences to identify genes, ESTs and repeat sequences, and to anchor the contig to the syntenic region of the human genome sequence (Hsap3q21-q22). The contig was further annotated based on this human/porcine comparative map, and was also anchored to the Sanger porcine framework map and the integrated map of Sscr13 by RH mapping. The annotated contig, containing 10 genes and 2 ESTs, showed a complete conservation of linkage (gene order and orientation) with the human genome sequence, based on 46 anchor points. This underlines the importance of the human/porcine comparative map for the identification of porcine genes associated with genetic defects and economically important traits, and for assembly of the porcine genome sequence.
ISSN:1471-2164
1471-2164
DOI:10.1186/1471-2164-6-133