In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean ( Phaseolus vulgaris L.)

( ) is a significant entomopathogenic fungus (EPF) in agriculture as a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and increased growth and yield....

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Published in:Plants (Basel) 2023-12, Vol.13 (1), p.22
Main Authors: Cavazos-Vallejo, Teodora, Valadez-Lira, José Alberto, Orozco-Flores, Alonso A, Gomez-Flores, Ricardo, Ek-Ramos, María Julissa, Quistián-Martínez, Deyanira, Alcocer-González, Juan Manuel, Tamez-Guerra, Patricia
Format: Article
Language:English
Subjects:
Beans
Beauveria bassiana
Biological control
Crops
Deoxyribonucleic acid
DNA
Endophytes
Entomopathogenic fungi
entomopathogenic fungus
Fungi
Genomes
group I introns
Host plants
Insects
Introns
Microorganisms
molecular detection
Monitoring
nested PCR
Pest control
Polymerase chain reaction
Soil microorganisms
Strains (organisms)
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Summary:( ) is a significant entomopathogenic fungus (EPF) in agriculture as a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and increased growth and yield. However, it is not known whether a strain has such a favorable impact on the plant, since it is a common soil microorganism. Therefore, techniques that allow strain monitoring will be advantageous. To date, methods for detecting or monitoring a specific EPF strain after external application are scarce. In the present study, an in planta nested PCR technique was standardized to differentiate between three strains (GHA, PTG4, and BB37) established as endophytes in bean plants under laboratory conditions by detecting the insertion profile of four group I introns located in the 28S gene of ribosomal DNA. This technique recognized a distinct pattern of bands of different sizes for each strain, with a sensitivity of 1 pg per 10 ng of plant DNA. This molecular approach may be more effective monitoring strains after application to evaluate their significance on crops.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants13010022