Development of a rat capnoperitoneum phantom to study drug aerosol deposition in the context of anticancer research on peritoneal carcinomatosis

• Published in: Scientific reports 2021-11, Vol.11 (1), p.21843-21843, Article 21843
• Main Authors: Göhler, Daniel, Geldner, Antje, Gritzki, Ralf, Lohse, Franz, Große, Stephan, Sobilo, Julien, Felsmann, Clemens, Buggisch, Jonathan R., Le Pape, Alain, Rudolph, Andreas, Stintz, Michael, Giger-Pabst, Urs
• Format: Article
• Language: English
• Subjects: 631/67/1059; 631/67/1059/153; 639/166/985; 639/301/930/12; 639/766/189; 639/766/419/1131; 692/308/1426; Aerosols; Animal models; Animals; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - pharmacokinetics; Chemotherapy; Computed tomography; Computer applications; Computer Simulation; Computer-Aided Design; Gastric cancer; Humanities and Social Sciences; Humans; Hydrodynamics; Injections, Intraperitoneal - instrumentation; Injections, Intraperitoneal - methods; Models, Animal; multidisciplinary; Peritoneal Neoplasms - diagnostic imaging; Peritoneal Neoplasms - drug therapy; Peritoneal Neoplasms - metabolism; Peritoneum; Peritoneum - diagnostic imaging; Peritoneum - metabolism; Phantoms, Imaging; Pressure; Rats; Rodents; Science; Science (multidisciplinary); Tomography, X-Ray Computed; User-Computer Interface
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-01332-0

Pressurized Intraperitoneal Aerosol Chemotherapy (PIPAC) is a promising approach with a high optimization potential for the treatment of peritoneal carcinomatosis. To study the efficacy of PIPAC and drugs, first rodent cancer models were developed. But inefficient drug aerosol supply and knowledge gaps concerning spatial drug distribution can limit the results based on such models. To study drug aerosol supply/deposition, computed tomography scans of a rat capnoperitoneum were used to deduce a virtual and a physical phantom of the rat capnoperitoneum (RCP). RCP qualification was performed for a specific PIPAC method, where the capnoperitoneum is continuously purged by the drug aerosol. In this context, also in-silico analyses by computational fluid dynamic modelling were conducted on the virtual RCP. The physical RCP was used for ex-vivo granulometric analyses concerning drug deposition. Results of RCP qualification show that aerosol deposition in a continuous purged rat capnoperitoneum depends strongly on the position of the inlet and outlet port. Moreover, it could be shown that the droplet size and charge condition of the drug aerosol define the deposition efficiency. In summary, the developed virtual and physical RCP enables detailed in-silico and ex-vivo analyses on drug supply/deposition in rodents.