A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions

Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work, we systematically analyzed every AGO using CRISPR-...

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Bibliographic Details
Published in:eLife 2023-02, Vol.12
Main Authors: Seroussi, Uri, Lugowski, Andrew, Wadi, Lina, Lao, Robert X, Willis, Alexandra R, Zhao, Winnie, Sundby, Adam E, Charlesworth, Amanda G, Reinke, Aaron W, Claycomb, Julie M
Format: Article
Language:English
Subjects:
22G-RNA
26G-RNA
Animals
Argonaute
Argonaute Proteins - genetics
Argonaute Proteins - metabolism
Caenorhabditis elegans - metabolism
Caenorhabditis elegans Proteins - metabolism
Developmental Biology
gene regulation
Gene Regulatory Networks
Genetics and Genomics
Genomes
Genomics
miRNA
Nematoda
piRNA
Proteins
RNA
RNA Interference
RNA, Small Interfering - metabolism
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Summary:Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work, we systematically analyzed every AGO using CRISPR-Cas9 genome editing to introduce GFP::3xFLAG tags. We have characterized the expression patterns of each AGO throughout development, identified small RNA binding complements, and determined the effects of loss on small RNA populations and developmental phenotypes. Our analysis indicates stratification of subsets of AGOs into distinct regulatory modules, and integration of our data led us to uncover novel stress-induced fertility and pathogen response phenotypes due to loss.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.83853