Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor

Chronic liver disease has become a major health problem that causes serious damage to human health. Since the existing treatment effect was not ideal, we need to seek new treatment methods. We utilized the gene recombination technology to obtain the human hair mesenchymal stem cells which overexpres...

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Bibliographic Details
Published in:Iranian journal of basic medical sciences 2017-06, Vol.20 (6), p.662-675
Main Authors: Zhou, Dan, Cheng, Hongjing, Liu, Jinyu, Zhang, Lei
Format: Article
Language:English
Subjects:
Growth factors
Hair follicle
HGF
Lentivirus
Liver diseases
Original
Skin & tissue grafts
Stem cells
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Summary:Chronic liver disease has become a major health problem that causes serious damage to human health. Since the existing treatment effect was not ideal, we need to seek new treatment methods. We utilized the gene recombination technology to obtain the human hair mesenchymal stem cells which overexpression of human hepatocyte growth factor (hHGF). Furthermore, we verified the property of transfected cells through detecting surface marker by flow cytometry. We show here establishment of the hHGF-overexpressing lentivirus vector, and successfully transfection to human hair follicle mesenchymal stem cells. The verified experiments could demonstrate the human hair follicle mesenchymal stem cells which have been transfected still have the properties of stem cells. We successfully constructed human hair follicle mesenchymal stem cells which overexpression hHGF, and maintain the same properties compared with pro-transfected cells.
ISSN:2008-3866
2008-3874
DOI:10.22038/IJBMS.2017.8834