Theoretical and Experimental Studies on the Evidence of 1,3-β-Glucan in Marennine of Haslea ostrearia

Marennine, a blue pigment produced by the blue diatom , is known to have some biological activities. This pigment is responsible for the greening of oysters on the West Coast of France. Other new species of blue diatom, , sp. inedit., sp. inedit, and , also produce marennine-like pigments with simil...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2023-07, Vol.28 (15), p.5625
Main Authors: Yusuf, Muhammad, Baroroh, Umi, Nuwarda, Rina Fajri, Prasetiya, Fiddy Semba, Ishmayana, Safri, Novianti, Mia Tria, Tohari, Taufik Ramdani, Hardianto, Ari, Subroto, Toto, Mouget, Jean-Luc, Pasetto, Pamela
Format: Article
Language:English
Subjects:
Bacteria
Carbohydrates
Cellulase
computational study
endo-1,3-β-glucanase
Enzymes
Glucose
Haslea ostrearia
hydrolysis
Hypotheses
marennine
Molecular structure
NMR
Nuclear magnetic resonance
Pigments
β-glucan
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Summary:Marennine, a blue pigment produced by the blue diatom , is known to have some biological activities. This pigment is responsible for the greening of oysters on the West Coast of France. Other new species of blue diatom, , sp. inedit., sp. inedit, and , also produce marennine-like pigments with similar biological activities. Aside from being a potential source of natural blue pigments, -like diatoms present a commercial potential for the aquaculture, food, cosmetics, and health industries. Unfortunately, for a hundred years, the exact molecular structure of this bioactive compound has remained a mystery. A lot of hypotheses regarding the chemical structure of marennine have been proposed. The recent discovery of this structure revealed that it is a macromolecule, mainly carbohydrates, with a complex composition. In this study, some glycoside hydrolases were used to digest marennine, and the products were further analyzed using nuclear magnetic resonance (NMR) and mass spectroscopy (MS). The reducing sugar assay showed that marennine was hydrolyzed only by endo-1,3-β-glucanase. Further insight into the structure of marennine was provided by the spectrum of H NMR, MS, a colorimetric assay, and a computational study, which suggest that the chemical structure of marennine contains 1,3-β-glucan.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28155625