Plant-expressed Zika virus envelope protein elicited protective immunity against the Zika virus in immunocompetent mice

Zika virus infection causes multiple clinical issues, including Guillain–Barré syndrome and neonatal malformation. Vaccination is considered as the only strategy for the prevention of ZIKV-induced clinical issues. This study developed a plant-based recombinant vaccine that transiently expressed the...

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Bibliographic Details
Published in:Scientific reports 2023-12, Vol.13 (1), p.22955-22955, Article 22955
Main Authors: Shin, Minna, Kang, Hyangju, Shin, Kyeong ryeol, Lee, Rangyeon, Kim, Kiju, Min, Kyungmin, Cho, Kyou-Nam, Sohn, Eun-Ju, Kim, Kwang Sung, Kim, Seok-Hyun, Cho, Yang Je, Park, Jeongho, Hahn, Tae-Wook
Format: Article
Language:English
Subjects:
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Aluminum
Animals
Antibodies
Antibodies, Neutralizing
Antibodies, Viral
Antigen presentation
Cell-mediated immunity
Cross-protection
Cytokines
Dengue fever
Env protein
Female
Genetic crosses
Guillain-Barre syndrome
Humanities and Social Sciences
Humoral immunity
Immunization
Immunocompetence
Infections
Mice
multidisciplinary
Neonates
Proteins
Science
Science (multidisciplinary)
Vaccines
Vector-borne diseases
Veterinary medicine
Viral envelope proteins
Viral Envelope Proteins - genetics
Viral Vaccines
Viruses
West Nile virus
Zika Virus
Zika Virus Infection
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Summary:Zika virus infection causes multiple clinical issues, including Guillain–Barré syndrome and neonatal malformation. Vaccination is considered as the only strategy for the prevention of ZIKV-induced clinical issues. This study developed a plant-based recombinant vaccine that transiently expressed the ZIKV envelope protein (ZikaEnv:aghFc) in Nicotiana benthamiana and evaluated the protective immunity afforded by it in immunocompetent mice. ZikaEnv:aghFc induced both humoral and cellular immunity at a low dose (1–5 μg). This immune-inducing potential was enhanced further when adjuvanted CIA09A. In addition, antigen-specific antibodies and neutralizing antibodies were vertically transferred from immunized females to their progeny and afforded both protective immunity to ZIKV and cross-protection to Dengue virus infection. These results suggest that our plant-based ZIKV vaccine provides a safe and efficient protective strategy with a competitive edge.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-47428-7