Peculiarities of impairments of absorptive-excretory and glycogen synthesis functions of the liver in the presence of acute blood loss complicated by limb ischemia-reperfusion and their correction by carbacetam

• Published in: Journal of education, health and sport health and sport, 2020-05, Vol.10 (5), p.301-314
• Main Authors: Horban, I. I., Hudyma, A. A., Maksymiv, R. V., Dzhavadova, N. A.
• Format: Article
• Language: English
• Subjects: blood loss; carbacetam; ischemia-reperfusion of the limb; liver
• ISSN: 2391-8306; 2391-8306
• DOI: 10.12775/JEHS.2020.10.05.032

Introduction. Under conditions of massive bleeding from the extremities, both in combat and in peacetime, the only means for salvation is applying tourniquet, which completely stops the arterial blood flow. It is believed that the maximum safe time for bleeding of the limb due to the use of the tourniquet is up to two hours. The systemic effect of acute blood loss complicated by ischemia-reperfusion of the limb on the liver, which is the main organ of detoxification of the body, remains almost unexplored.Objective of research: to determine the effect of acute blood loss complicated by two-hour limb ischemia and its reperfusion on the absorption-excretory and glycogen-synthesizing function of the liver.Materials of the research and their discussion. The experiments were performed on 108 nonlinear male rats weighing 200-220 g. All animals were divided into four groups: control and three experimental. All interventions were performed under thiopental-sodium anesthesia. In the first experimental group, the animals were simulated ischemia-reperfusion of the limb by applying a strip of elastic harness "SWAT-T" (USA)10 mm wide for 120 minutes proximal to the left paw. In the second experimental group, acute blood loss (20% of the circulating blood volume) was simulated by cutting the femoral vein. In the third experimental group, these injuries were combined. In the control group, the animals were anesthetized, and then taken for research after 1 hour.After 2 and 3 hours, as well as after 1, 7 and 14 days in experimental animals were determined by the absorption-excretory and glycogen-synthesizing functions of the liver.Result of the research and their discussion. It was found that the simulation of two-hour limb ischemia after 3 h and 1 day of reperfusion is accompanied by a significant decrease in hepatic excretory function, accompanied by a slowing of bile bromosulfalein excretion and its normalization starting from 7 days of the experiment. Under conditions of acute blood loss, the disorders deepen at all times of observation. Complications of acute blood loss by ischemia-reperfusion of the limb, contributes to a greater slowing of the absorption and excretory function of the liver compared to the simulation of only acute blood loss. The result was statistically significant after 7 and 14 days of the experiment.With regard to the content of glycogen in the liver, it was found that the simulation of only ischemia-reperfusion of the limb was accompanied by a si