Caprate Modulates Intestinal Barrier Function in Porcine Peyer's Patch Follicle-Associated Epithelium

Many food components influence intestinal epithelial barrier properties and might therefore also affect susceptibility to the development of food allergies. Such allergies are triggered by increased antibody production initiated in Peyer's patches (PP). Usually, the presentation of antigens in...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2019-03, Vol.20 (6), p.1418
Main Authors: Radloff, Judith, Cornelius, Valeria, Markov, Alexander G, Amasheh, Salah
Format: Article
Language:English
Subjects:
Cell culture
claudins
Coconut oil
Epithelium
Experiments
Fatty acids
Food allergies
Glycerol
Immunology
Intestine
Laboratory animals
Low concentrations
Milk
Nutrients
Nutrition
Pancreas
Permeability
Proteins
tight junction
tissue barrier
tricellulin
Triglycerides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Many food components influence intestinal epithelial barrier properties and might therefore also affect susceptibility to the development of food allergies. Such allergies are triggered by increased antibody production initiated in Peyer's patches (PP). Usually, the presentation of antigens in the lumen of the gut to the immune cells of the PP is strongly regulated by the follicle-associated epithelium (FAE) that covers the PP. As the food component caprate has been shown to impede barrier properties in villous epithelium, we hypothesized that caprate also affects the barrier function of the PP FAE, thereby possibly contributing a risk factor for the development of food allergies. In this study, we have focused on the effects of caprate on the barrier function of PP, employing in vitro and ex vivo experimental setups to investigate functional and molecular barrier properties. Incubation with caprate induced an increase of transepithelial resistance, and a marked increase of permeability for the paracellular marker fluorescein in porcine PP to 180% of control values. These effects are in accordance with changes in the expression levels of the barrier-forming tight junction proteins tricellulin and claudin-5. This barrier-affecting mechanism could be involved in the initial steps of a food allergy, since it might trigger unregulated contact of the gut lumen with antigens.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20061418