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A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable laboratory techniques is important to allow a proper case management and impleme...
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| Published in: | Memórias do Instituto Oswaldo Cruz 2016-06, Vol.111 (6), p.385-390 |
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| container_title | Memórias do Instituto Oswaldo Cruz |
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| creator | Tolardo, Aline Lavado Souza, William Marciel de Romeiro, Marilia Farignoli Vieira, Luiz Carlos Souza Luna, Luciano Kleber de Henriques, Dyana Alves Araujo, Jansen de Hassegawa Siqueira, Carlos Eduardo Colombo, Tatiana Elias Aquino, Victor Hugo Fonseca, Benedito Antonio Lopes da Bronzoni, Roberta Vieira de Morais Nogueira, Maurício Lacerda Durigon, Edison Luiz Figueiredo, Luiz Tadeu Moraes |
| description | Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular
stomatitis disease in cattle, horses and pigs, as well as sporadic
human cases of acute febrile illness. Therefore, diagnosis of VSV
infections by reliable laboratory techniques is important to allow a
proper case management and implementation of strategies for the
containment of virus spread. We show here a sensitive and reproducible
real-time reverse transcriptase polymerase chain reaction (RT-PCR) for
detection and quantification of VSV. The assay was evaluated with
arthropods and serum samples obtained from horses, cattle and patients
with acute febrile disease. The real-time RT-PCR amplified the Piry,
Carajas, Alagoas and Indiana Vesiculovirus at a melting temperature
81.02 ± 0.8ºC, and the sensitivity of assay was estimated in
10 RNA copies/mL to the Piry Vesiculovirus. The viral genome has been
detected in samples of horses and cattle, but not detected in human
sera or arthropods. Thus, this assay allows a preliminary differential
diagnosis of VSV infections. |
| doi_str_mv | 10.1590/0074-02760150456 |
| format | article |
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stomatitis disease in cattle, horses and pigs, as well as sporadic
human cases of acute febrile illness. Therefore, diagnosis of VSV
infections by reliable laboratory techniques is important to allow a
proper case management and implementation of strategies for the
containment of virus spread. We show here a sensitive and reproducible
real-time reverse transcriptase polymerase chain reaction (RT-PCR) for
detection and quantification of VSV. The assay was evaluated with
arthropods and serum samples obtained from horses, cattle and patients
with acute febrile disease. The real-time RT-PCR amplified the Piry,
Carajas, Alagoas and Indiana Vesiculovirus at a melting temperature
81.02 ± 0.8ºC, and the sensitivity of assay was estimated in
10 RNA copies/mL to the Piry Vesiculovirus. The viral genome has been
detected in samples of horses and cattle, but not detected in human
sera or arthropods. Thus, this assay allows a preliminary differential
diagnosis of VSV infections.</description><identifier>ISSN: 1678-8060</identifier><identifier>ISSN: 0074-0276</identifier><identifier>EISSN: 1678-8060</identifier><identifier>DOI: 10.1590/0074-02760150456</identifier><identifier>PMID: 27276185</identifier><language>eng</language><publisher>Brazil: Fundação Oswaldo Cruz, Fiocruz</publisher><subject>Animals ; Cattle ; diagnosis of vesicular stomatitis ; Horses - virology ; Humans ; PARASITOLOGY ; quantitative real-time RT-PCR ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - genetics ; Sensitivity and Specificity ; TROPICAL MEDICINE ; Vesicular Stomatitis - diagnosis ; Vesiculovirus ; Vesiculovirus - genetics ; zoonotic virus</subject><ispartof>Memórias do Instituto Oswaldo Cruz, 2016-06, Vol.111 (6), p.385-390</ispartof><rights>Copyright 2016 - Memórias do Instituto Oswaldo Cruz</rights><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b540t-18ba765972203b8bb9e8e0e14ee33e49720b0c8868dc07a7fcef2252872e0ad33</citedby><cites>FETCH-LOGICAL-b540t-18ba765972203b8bb9e8e0e14ee33e49720b0c8868dc07a7fcef2252872e0ad33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909037/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909037/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,24150,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27276185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tolardo, Aline Lavado</creatorcontrib><creatorcontrib>Souza, William Marciel de</creatorcontrib><creatorcontrib>Romeiro, Marilia Farignoli</creatorcontrib><creatorcontrib>Vieira, Luiz Carlos</creatorcontrib><creatorcontrib>Souza Luna, Luciano Kleber de</creatorcontrib><creatorcontrib>Henriques, Dyana Alves</creatorcontrib><creatorcontrib>Araujo, Jansen de</creatorcontrib><creatorcontrib>Hassegawa Siqueira, Carlos Eduardo</creatorcontrib><creatorcontrib>Colombo, Tatiana Elias</creatorcontrib><creatorcontrib>Aquino, Victor Hugo</creatorcontrib><creatorcontrib>Fonseca, Benedito Antonio Lopes da</creatorcontrib><creatorcontrib>Bronzoni, Roberta Vieira de Morais</creatorcontrib><creatorcontrib>Nogueira, Maurício Lacerda</creatorcontrib><creatorcontrib>Durigon, Edison Luiz</creatorcontrib><creatorcontrib>Figueiredo, Luiz Tadeu Moraes</creatorcontrib><title>A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus</title><title>Memórias do Instituto Oswaldo Cruz</title><addtitle>Mem Inst Oswaldo Cruz</addtitle><description>Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular
stomatitis disease in cattle, horses and pigs, as well as sporadic
human cases of acute febrile illness. Therefore, diagnosis of VSV
infections by reliable laboratory techniques is important to allow a
proper case management and implementation of strategies for the
containment of virus spread. We show here a sensitive and reproducible
real-time reverse transcriptase polymerase chain reaction (RT-PCR) for
detection and quantification of VSV. The assay was evaluated with
arthropods and serum samples obtained from horses, cattle and patients
with acute febrile disease. The real-time RT-PCR amplified the Piry,
Carajas, Alagoas and Indiana Vesiculovirus at a melting temperature
81.02 ± 0.8ºC, and the sensitivity of assay was estimated in
10 RNA copies/mL to the Piry Vesiculovirus. The viral genome has been
detected in samples of horses and cattle, but not detected in human
sera or arthropods. Thus, this assay allows a preliminary differential
diagnosis of VSV infections.</description><subject>Animals</subject><subject>Cattle</subject><subject>diagnosis of vesicular stomatitis</subject><subject>Horses - virology</subject><subject>Humans</subject><subject>PARASITOLOGY</subject><subject>quantitative real-time RT-PCR</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reproducibility of Results</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - genetics</subject><subject>Sensitivity and Specificity</subject><subject>TROPICAL MEDICINE</subject><subject>Vesicular Stomatitis - diagnosis</subject><subject>Vesiculovirus</subject><subject>Vesiculovirus - genetics</subject><subject>zoonotic virus</subject><issn>1678-8060</issn><issn>0074-0276</issn><issn>1678-8060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVUk1r3DAQNaWlSdPeeyo-9uJ0JFsfvhRCSNtAoId-XIUkjxMtsrSR7IX8-8rZzTYBgWae5r0ZDa-qPhI4J6yHLwCia4AKDoRBx_ir6pRwIRsJHF4_i0-qdzlvoFS2vHtbnVBROESy02q6qBNq38xuwhLtMGWs56RDtsltZ12ybfQPE6Y1tHfahZVgZxdDPcZUDzjjPtNhqO8XHWY3OqsfoTjWfzE7u_i4c2nJ76s3o_YZPxzus-rPt6vflz-am5_fry8vbhrDOpgbIo0WnPWCUmiNNKZHiYCkQ2xb7AoOBqyUXA4WhBajxZFSRqWgCHpo27Pqeq87RL1R2-QmnR5U1E49AjHdKp1mZz0qQ8koRm7ZwNpOSlbaCdSG9Byl1UYXrfO9VrYOfVSbuKRQhle_1u2rdfsUCAcoe4ZWskL4uidsFzPhYDGUffoXU7x8Ce5O3cad6nrooRVF4PNBIMX7BfOsJpcteq8DxiUrInohOWeyL6WwL7Up5pxwPLYhoFaLqOOQB4sUyqfn4x0JT574_2HjoncBjxXFEVo9gdGWU75dFP8BhRLICg</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Tolardo, Aline Lavado</creator><creator>Souza, William Marciel de</creator><creator>Romeiro, Marilia Farignoli</creator><creator>Vieira, Luiz Carlos</creator><creator>Souza Luna, Luciano Kleber de</creator><creator>Henriques, Dyana Alves</creator><creator>Araujo, Jansen de</creator><creator>Hassegawa Siqueira, Carlos Eduardo</creator><creator>Colombo, Tatiana Elias</creator><creator>Aquino, Victor Hugo</creator><creator>Fonseca, Benedito Antonio Lopes da</creator><creator>Bronzoni, Roberta Vieira de Morais</creator><creator>Nogueira, Maurício Lacerda</creator><creator>Durigon, Edison Luiz</creator><creator>Figueiredo, Luiz Tadeu Moraes</creator><general>Fundação Oswaldo Cruz, Fiocruz</general><general>Instituto Oswaldo Cruz, Ministério da Saúde</general><general>Fundação Oswaldo Cruz (FIOCRUZ)</general><scope>RBI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>GPN</scope><scope>DOA</scope></search><sort><creationdate>20160601</creationdate><title>A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus</title><author>Tolardo, Aline Lavado ; Souza, William Marciel de ; Romeiro, Marilia Farignoli ; Vieira, Luiz Carlos ; Souza Luna, Luciano Kleber de ; Henriques, Dyana Alves ; Araujo, Jansen de ; Hassegawa Siqueira, Carlos Eduardo ; Colombo, Tatiana Elias ; Aquino, Victor Hugo ; Fonseca, Benedito Antonio Lopes da ; Bronzoni, Roberta Vieira de Morais ; Nogueira, Maurício Lacerda ; Durigon, Edison Luiz ; Figueiredo, Luiz Tadeu Moraes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b540t-18ba765972203b8bb9e8e0e14ee33e49720b0c8868dc07a7fcef2252872e0ad33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>diagnosis of vesicular stomatitis</topic><topic>Horses - virology</topic><topic>Humans</topic><topic>PARASITOLOGY</topic><topic>quantitative real-time RT-PCR</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Reproducibility of Results</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - genetics</topic><topic>Sensitivity and Specificity</topic><topic>TROPICAL MEDICINE</topic><topic>Vesicular Stomatitis - diagnosis</topic><topic>Vesiculovirus</topic><topic>Vesiculovirus - genetics</topic><topic>zoonotic virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tolardo, Aline Lavado</creatorcontrib><creatorcontrib>Souza, William Marciel de</creatorcontrib><creatorcontrib>Romeiro, Marilia Farignoli</creatorcontrib><creatorcontrib>Vieira, Luiz Carlos</creatorcontrib><creatorcontrib>Souza Luna, Luciano Kleber de</creatorcontrib><creatorcontrib>Henriques, Dyana Alves</creatorcontrib><creatorcontrib>Araujo, Jansen de</creatorcontrib><creatorcontrib>Hassegawa Siqueira, Carlos Eduardo</creatorcontrib><creatorcontrib>Colombo, Tatiana Elias</creatorcontrib><creatorcontrib>Aquino, Victor Hugo</creatorcontrib><creatorcontrib>Fonseca, Benedito Antonio Lopes da</creatorcontrib><creatorcontrib>Bronzoni, Roberta Vieira de Morais</creatorcontrib><creatorcontrib>Nogueira, Maurício Lacerda</creatorcontrib><creatorcontrib>Durigon, Edison Luiz</creatorcontrib><creatorcontrib>Figueiredo, Luiz Tadeu Moraes</creatorcontrib><collection>Bioline International Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SciELO</collection><collection>Directory of Open Access Journals(OpenAccess)</collection><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tolardo, Aline Lavado</au><au>Souza, William Marciel de</au><au>Romeiro, Marilia Farignoli</au><au>Vieira, Luiz Carlos</au><au>Souza Luna, Luciano Kleber de</au><au>Henriques, Dyana Alves</au><au>Araujo, Jansen de</au><au>Hassegawa Siqueira, Carlos Eduardo</au><au>Colombo, Tatiana Elias</au><au>Aquino, Victor Hugo</au><au>Fonseca, Benedito Antonio Lopes da</au><au>Bronzoni, Roberta Vieira de Morais</au><au>Nogueira, Maurício Lacerda</au><au>Durigon, Edison Luiz</au><au>Figueiredo, Luiz Tadeu Moraes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus</atitle><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle><addtitle>Mem Inst Oswaldo Cruz</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>111</volume><issue>6</issue><spage>385</spage><epage>390</epage><pages>385-390</pages><issn>1678-8060</issn><issn>0074-0276</issn><eissn>1678-8060</eissn><abstract>Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular
stomatitis disease in cattle, horses and pigs, as well as sporadic
human cases of acute febrile illness. Therefore, diagnosis of VSV
infections by reliable laboratory techniques is important to allow a
proper case management and implementation of strategies for the
containment of virus spread. We show here a sensitive and reproducible
real-time reverse transcriptase polymerase chain reaction (RT-PCR) for
detection and quantification of VSV. The assay was evaluated with
arthropods and serum samples obtained from horses, cattle and patients
with acute febrile disease. The real-time RT-PCR amplified the Piry,
Carajas, Alagoas and Indiana Vesiculovirus at a melting temperature
81.02 ± 0.8ºC, and the sensitivity of assay was estimated in
10 RNA copies/mL to the Piry Vesiculovirus. The viral genome has been
detected in samples of horses and cattle, but not detected in human
sera or arthropods. Thus, this assay allows a preliminary differential
diagnosis of VSV infections.</abstract><cop>Brazil</cop><pub>Fundação Oswaldo Cruz, Fiocruz</pub><pmid>27276185</pmid><doi>10.1590/0074-02760150456</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1678-8060 |
| ispartof | Memórias do Instituto Oswaldo Cruz, 2016-06, Vol.111 (6), p.385-390 |
| issn | 1678-8060 0074-0276 1678-8060 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_b21f7f6c5d534885b8b7eab196e8caba |
| source | SciELO Brazil; PubMed Central |
| subjects | Animals Cattle diagnosis of vesicular stomatitis Horses - virology Humans PARASITOLOGY quantitative real-time RT-PCR Real-Time Polymerase Chain Reaction Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics Sensitivity and Specificity TROPICAL MEDICINE Vesicular Stomatitis - diagnosis Vesiculovirus Vesiculovirus - genetics zoonotic virus |
| title | A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus |
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