A systemic approach to identify non-abundant immunogenic proteins in Lyme disease pathogens

The present manuscript employed a systemic approach to identify non-abundant proteins in cultured that are otherwise masked or hidden due to the overwhelming presence of abundant Osps like OspA, OspB, and OspC. As these Osps are either absent or transiently expressed in mammals, we performed a proof...

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Bibliographic Details
Published in:mSystems 2024-01, Vol.9 (1), p.e0108723
Main Authors: Yaş, Ozlem Buyuktanir, Coleman, Adam S, Lipman, Rachel M, Sharma, Kavita, Raghunandanan, Sajith, Alanazi, Fuad, Rana, Vipin S, Kitsou, Chrysoula, Yang, Xiuli, Pal, Utpal
Format: Article
Language:English
Subjects:
Animal models
Antigens
Bacteriology
Borrelia burgdorferi
Borreliosis
Deficient mutant
Gene expression
Genetic markers
Immunogenicity
Infections
Liquid chromatography
Lyme disease
Mass spectroscopy
Membrane proteins
Microscopy
mutagenesis
Observation
OspA protein
Outer surface proteins
Pathogens
Proteins
Spirochetes
Vaccines
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Summary:The present manuscript employed a systemic approach to identify non-abundant proteins in cultured that are otherwise masked or hidden due to the overwhelming presence of abundant Osps like OspA, OspB, and OspC. As these Osps are either absent or transiently expressed in mammals, we performed a proof-of-concept study in which their removal allowed the analysis of otherwise less abundant antigens in OspABC-deficient mutants and identified several immunogenic proteins, including BBA34 and BB0238. These antigens could serve as novel vaccine candidates and/or genetic markers of Lyme borreliosis, promoting new research in the clinical diagnosis and prevention of Lyme disease.
ISSN:2379-5077
2379-5077
DOI:10.1128/msystems.01087-23