Towards the Control of the Reproduction of the Yellow Clam Amarilladesma mactroides (Reeve, 1854) in Captivity: Effects of Different Stimuli on the Spawning of Laboratory-Conditioned and Unconditioned Breeders

• Published in: Fishes 2023-01, Vol.8 (1), p.37
• Main Authors: Gauthier, Nathália Byrro, Góes, Filipe Scavone, Pedrosa, Virgínia Fonseca, Roselet, Fábio, Romano, Luis Alberto, Cavalli, Ronaldo Olivera
• Format: Article
• Language: English
• Subjects: Alkalinity; Amarilladesma mactroides; aquaculture; bivalve; Captivity; Clams; Condition factor; conditioning; Developmental stages; gonadal development; Gonads; Homogenization; Hydrogen peroxide; Laboratories; Larvae; maturation; mollusk; Mollusks; Salinity; Seawater; Sexual maturity; Spawning; Sperm; Spermatozoa; Stimuli; Temperature effects; Wildlife conservation
• ISSN: 2410-3888; 2410-3888
• DOI: 10.3390/fishes8010037

The effects of temperature manipulation, addition of sperm solution, and exposure to alkalized pH and/or hydrogen peroxide (H2O2) as possible spawning inducers in laboratory-conditioned and unconditioned adults of the yellow clam (Amarilladesma mactroides) were evaluated. In three trials, clams were laboratory-conditioned for 14 days and exposed to thermal shocks (from 20 °C to 23–29 °C), while clams from three additional trials were not conditioned but acclimatized for 45 min before spawning induction. Although conditioning advanced gonad maturity and increased the condition factor, none of the thermal treatments triggered spawning in these first trials. Histological analysis indicated that the gonads of conditioned clams were not mature. Alternatively, unfertilized and fertilized eggs, and larvae were observed after unconditioned clams were induced to spawn. The gonads of unconditioned clams were in an advanced stage of maturity. Exposure to temperature shocks, alone or in combination with the addition of sperm solution and with H2O2, resulted in spawning. Clams exposed to H2O2 kept their valves closed and had a high mortality rate. Temperature manipulation is the most promising stimulus to induce spawning in A. mactroides. As the success of conditioning depends on the stage of gonadal development at the time of capture in the wild, a period longer than 14 days may be necessary.