Gonadotropins facilitate potential differentiation of human bone marrow mesenchymal stem cells into Leydig cells in vitro

Abstract Infertility due to low testosterone levels has increased in recent years. This has impacted the social well-being of the patients. This study was undertaken to investigate the potential of gonadotropins in facilitating differentiation of human bone marrow mesenchymal stem cells (BMSCs) into...

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Published in:The Kaohsiung journal of medical sciences 2016-01, Vol.32 (1), p.1-9
Main Authors: Hou, Lin, Dong, Qiang, Wu, Yun-Jian, Sun, Yuan-Xing, Guo, Yan-Yu, Huo, Yue-Hong
Format: Article
Language:English
Subjects:
3-Hydroxysteroid Dehydrogenases - metabolism
Adult
Bone Marrow Cells - physiology
Cell Differentiation
Cell Proliferation
Cell Shape
Cells, Cultured
Chorionic Gonadotropin - physiology
Female
Gonadotropins
Human bone marrow mesenchymal stem cells
Humans
Internal Medicine
Leydig cells
Leydig Cells - enzymology
Leydig Cells - physiology
Luteinizing Hormone - physiology
Male
Menotropins - physiology
Mesenchymal Stromal Cells - physiology
Testosterone
Testosterone - biosynthesis
Young Adult
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Summary:Abstract Infertility due to low testosterone levels has increased in recent years. This has impacted the social well-being of the patients. This study was undertaken to investigate the potential of gonadotropins in facilitating differentiation of human bone marrow mesenchymal stem cells (BMSCs) into Leydig cells in vitro . BMSCs were isolated, cultured, and their biological characteristics were observed. BMSCs were induced with gonadotropins in vitro and their ability to differentiate into Leydig cells was studied. The level of expression of 3-beta hydroxysteroid dehydrogenase (3β-HSD) and secretion of testosterone were determined using flow cytometry and enzyme-linked immunosorbent assay, respectively, and the results were compared between the experimental and control groups. The cultured BMSCs showed a typical morphology of the fibroblast-like colony. The growth curve of cells formed an S-shape. After inducing the cells for 8–13 days, the cells in the experimental group increased in size and showed typical characteristics of Leydig cells, and the growth occurred in spindle or stellate shapes. Cells from the experimental group highly expressed 3β-HSD, and there was a gradual increase in the number of Leydig cells. The control group did not express 3β-HSD. The level of testosterone in the experimental group was higher than the control group ( p  
ISSN:1607-551X
2410-8650
DOI:10.1016/j.kjms.2015.10.008