Genetic inhibition of CARD9 accelerates the development of atherosclerosis in mice through CD36 dependent-defective autophagy

• Published in: Nature communications 2023-08, Vol.14 (1), p.4622-4622, Article 4622
• Main Authors: Zhang, Yujiao, Vandestienne, Marie, Lavillegrand, Jean-Rémi, Joffre, Jeremie, Santos-Zas, Icia, Lavelle, Aonghus, Zhong, Xiaodan, Le Goff, Wilfried, Guérin, Maryse, Al-Rifai, Rida, Laurans, Ludivine, Bruneval, Patrick, Guérin, Coralie, Diedisheim, Marc, Migaud, Melanie, Puel, Anne, Lanternier, Fanny, Casanova, Jean-Laurent, Cochain, Clément, Zernecke, Alma, Saliba, Antoine-Emmanuel, Mokry, Michal, Silvestre, Jean-Sebastien, Tedgui, Alain, Mallat, Ziad, Taleb, Soraya, Lenoir, Olivia, Vindis, Cécile, Camus, Stéphane M., Sokol, Harry, Ait-Oufella, Hafid
• Format: Article
• Language: English
• Subjects: 13/2; 13/21; 13/31; 13/51; 14/1; 38; 38/88; 38/91; 631/250/2504/342; 631/80/39; 692/4019/592/75; Adaptive systems; Animal models; Animals; Apolipoprotein E; Apolipoproteins E - genetics; Arteriosclerosis; Atherosclerosis; Atherosclerosis - metabolism; Autophagy; Autophagy - genetics; CARD Signaling Adaptor Proteins - metabolism; Caspase; CD36 antigen; Cell death; Deletion; Humanities and Social Sciences; Humans; Immune system; Inflammation; Life Sciences; Lipids; Macrophages; Metformin; Mice; Mice, Inbred C57BL; Mice, Knockout; Monocytes; multidisciplinary; Phenotypes; RAG2 protein; Rapamycin; Science; Science (multidisciplinary); Signal transduction; Transcriptomics
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-40216-x

Caspase recruitment-domain containing protein 9 (CARD9) is a key signaling pathway in macrophages but its role in atherosclerosis is still poorly understood. Global deletion of Card9 in Apoe -/- mice as well as hematopoietic deletion in Ldlr -/- mice increases atherosclerosis. The acceleration of atherosclerosis is also observed in Apoe -/- Rag2 -/- Card9 -/- mice, ruling out a role for the adaptive immune system in the vascular phenotype of Card9 deficient mice. Card9 deficiency alters macrophage phenotype through CD36 overexpression with increased IL-1β production, increased lipid uptake, higher cell death susceptibility and defective autophagy. Rapamycin or metformin, two autophagy inducers, abolish intracellular lipid overload, restore macrophage survival and autophagy flux in vitro and finally abolish the pro-atherogenic effects of Card9 deficiency in vivo. Transcriptomic analysis of human CARD9 -deficient monocytes confirms the pathogenic signature identified in murine models. In summary, CARD9 is a key protective pathway in atherosclerosis, modulating macrophage CD36-dependent inflammatory responses, lipid uptake and autophagy. Previous studies suggested a role for CARD9 pathway in atherosclerosis but the underlying mechanisms remain poorly understood. Here, the authors show that the pro-atherogenic effects of Card9 deficiency are mediated by CD36-dependent defective autophagy that can be reversed by rapamycin and metformin.