The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication

To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We...

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Bibliographic Details
Published in:Poultry science 2022-06, Vol.101 (6), p.101863-101863, Article 101863
Main Authors: Wan, Jieyu, Li, Fangjie, Wang, Mingshu, Cheng, Anchun, Tian, Bin, Yang, Qiao, Wu, Ying, Ou, Xumin, Mao, Sai, Sun, Di, Zhang, Shaqiu, Huang, Juan, Gao, Qun, Zhao, XinXin, Chen, Shun, Liu, Mafeng, Jia, Renyong, Zhu, Dekang
Format: Article
Language:English
Subjects:
duck enteritis virus
IMMUNOLOGY, HEALTH AND DISEASE
UL14
virion morphology
virus replication
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Summary:To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We found that the CHv-BAC-ΔUL14 viruses exhibited impaired virion morphogenesis in transmission electron microscopy (TEM) studies. Furthermore, CHv-BAC-ΔUL14 exhibited a plaque size reduction in duck embryo fibroblasts (DEFs). Finally, CHv-BAC-ΔUL14 exhibited a significant viral growth defect. Taken together, our findings suggest that DPV UL14 protein regulates viral morphogenesis for efficient viral replication.
ISSN:0032-5791
1525-3171
DOI:10.1016/j.psj.2022.101863